1995 Fiscal Year Final Research Report Summary
ISOLATION AND CHARACTERIZATION OF A NOVEL HUMAN SPERM ANTIGEN (H-SP-1)
| Project/Area Number |
06671697
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | HYOGO COLLEGE OF MEDICINE |
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Principal Investigator |
KOMORI Shinji HYOGO COLLEGE OF MEDICINE,DEPARTMENT OF OBSTETRICS AND GYNECOLOGY,ASSISTANT PROFESSOR, 医学部, 助手 (60195865)
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| Co-Investigator(Kenkyū-buntansha) |
SAWAI Hideaki HYOGO COLLEGE OF MEDICINE,DEPARTMENT OF OBSTETRICS AND GYNECOLOGY,ASSISTANT PROF, 医学部, 助手 (80215904)
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| Project Period (FY) |
1994 – 1995
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| Keywords | sperm antigen / calcium dependent signal transduction |
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| Research Abstract |
We isolated a human sperm antigen gene (h-sp-1) by screening from human testis cDNA library with human sperm specific antisera. The sequence analysis of the h-sp-1 gene indicated that it contained 2170 bp and had 45% homology to human synaptophysin gene, which was considered to play a crucial role in calcium dependent signal transduction in synaptic vesicles in the terminal end of nerves. The analysis of the deduced amino acids from the h-sp-1 gene showed that it encoded 259 amino acids containing four hydrophilic regions and four hydrophobic regions. This indicated that the molecules encoded by the h-sp-1 gene might be a transmembrane protein with four membrane spanning regions like the synaptophysin. In order to characterize the molecules encoded by the hsp-1 gene, the antisera were generated by immunizing rabbits and mice with synthetic polypeptides (No174-198) based on the h-sp-1 gene. The resultant antisera stained fixed human sperm head by indirect immunofluorescence staining. The purified anti-h-sp-1 antibodies showed the inhibitory effect on human sperm penetration assay into zona-free hamster eggs. However they did not show any sperm immobilizing and agglutinating activities. These results suggested that the molecules encoded by the h-sp-1 gene might play an important role in the sperm-egg interaction.
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