Biomaterials incorporating releasable growth factor

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 06680854
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: TABATA Yasuhiko Kyoto University, Res.Ctr.for Biomed.Eng., Associate Professor, 生体医療工学研究センター, 助教授 (50211371)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Toshio University of Osaka Prefecture, Res.Inst.for Adv.Sci.and Tech., Professor, 先端科学研究所, 教授 (90026089) ; IKADA Yoshito Kyoto Univ, Res.Ctr.for Biomed.Eng., Professor, 生体医療工学研究センター, 教授 (00025909)
• Project Period (FY): 1994 – 1996
• Keywords: Growth factor / Gelatin / Hydrogel / Release / Neovascularization / Biodegradation / bFGF

Research Abstract

Basic fibroblast growth factor (bFGF) was impregnated through polyin complexation into biodegradable hydrogels prepared by crosslinking of acidic gelatin with the isoelectric point of 4.9. Release experiments demonstrated not to release bFGF from the gelatin hydrogel under the in vitro non-degradation conditions. The hydrogels were degraded with time following their subcutaneous implantation on the mouse back and the period of in vivo degradation varied depending on the water content of hydrogels. The gelatin hydrogels containing bFGF strongly induced neovascularization around the implantation site, whereas subcutaneous injection of bFGF in the solution form did not increase the amount of tissue hemoglobin and the wet weight of tissue. The bFGF-impregnated gelatin hydrogel of lower water content retained the vascularization effect for a longer period. It was concluded that bFGF was released from gelatin hydrogel as a result of their in vivo degradation, leading to more efficient induction of neovascularization than free bFGF without impregnation in the hydrogel.