Immunological Aspect for Adversed Reaction of Contrast Media

1995 Fiscal Year Final Research Report Summary

• Project/Area Number: 06807075
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Juntendo University School of Medicine
• Principal Investigator: MIYAUCHI Teruyuki Juntendo University School of Medicine, Radiology, 医学部, 助手 (90219725)
• Co-Investigator(Kenkyū-buntansha): OKUMURA Koh Juntendo University School of Medicine, Immunology, Prof., 医学部, 教授 (50009700) ; HIRANO Takao Juntendo University School of Medicine, Serology, Assist. Prof., 医学部, 助教授 (10165186) ; KATAYAMA Hitoshi Juntendo University School of Medicine, Radiology, Prof., 医学部, 教授 (80053007)
• Project Period (FY): 1994 – 1995
• Keywords: Iodimated Contrast Media / Adversed Reaction / Interleukin-4

Research Abstract

Various side effects have been associated with the clinical use of contrast media. Immunological mechanisms have been proposed ; however there were very few experimental studies with animal models. We attempted to develop murine models to know whether anaphylactic antibodies such as IgE and IgG1 against hapten (DNP) were enhanced with contrast media (Iopamidol) as an adjuvant or contrast media (Iopamidol) produced antibody of the IgE class. The results showed that anti-hapten IgE and IgG1 productions were greatly enhanced with immunogen plus contrast media (Iopamidol). Anti-contrast media (Iopamidol) antibody of the IgE class could not be detected by PCA reactions. The enhancement of IgE and IgG1 productions for hapten were related with IL-4 release by neutralization test used by monoclonal anti-IL-4 antibody. This is the first observation to show that contrast media may have a strong adjuvant effect for productions of IgE and IgG1. This murine model demonstrates a possible immunological function of contrast media in vivo.