| Research Abstract |
Research results are summarized as follows.
1. Establishment of an in vitro experimental system for evaluation of role of dexamethasone, extracellular matrix proteins, and TGF-beta in regulation of osteoblastic differentiation, using anewly developed human osteoblastic cell line, SV-HFO.
2. Detection of bone sialoprotein in bone-derived malignancies using newly developed monoclonal antibodies.
3. Experimental therapy of osteosarcoma with a combination of a chemotherapeutic reagent, MTX, and B7-transfected tumor vaccine in a rat orthotopic implantation model.
4. Demonstration of an inhibitory role of hsp9O in regulation of growth of a human osteosarcoma cell line, BOS.
5. Induction of MMP-9 and high metastatic phenotype in lines of human and mouse malignant tumor cells by transfection of ElAF, a member of eta oncogene fanily.
6. Demonstration of presence of concomitant tumor resistance, an inhibitory effect of the primary lesion on metastatic tumor growth, in a rat orthotopic tumor implantation model of osteosarcoma.
7. Demonstration of correlation between lack of erbB2 expression and poor prognosis in osteosarcoma patients.
8. Augmentation of anti-tumor immunity by inhibition of AICD, activation-induced cell death, of lymphokine-activated killer cells by pretreatmen with N-acetyl cysteine.
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