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1996 Fiscal Year Final Research Report Summary

RISK ASSESSMENT OF ENVIRONMENTAL MICROPOLLUTANTS BY USING DNA STRAND BREAKS IN HUMAN CELLS

Research Project

Project/Area Number 07455211
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MATSUI Saburo  KYOTO UNIVERSITY,ENGINEERING,PROFESSOR, 工学部, 教授 (90092808)

Co-Investigator(Kenkyū-buntansha) TAKEBE Hiraku  KYOTO UNIVERSITY,MEDICINE,PROFESSOR, 医学部, 教授 (10028318)
MATSUDA Tomonari  KYOTO UNIVERSITY,ENGINEERING,ASSISTANT, 工学部, 助手 (50273488)
YAMADA Harumi  KYOTO UNIVERSITY,ENGINEERING,ASSISTANT, 工学部, 助手 (40089123)
Project Period (FY) 1995 – 1996
KeywordsHUMANCELLS / DNA STRAND BREAKS / DNA DAMAGE / CHLORINATED ORGANO COMPOUNDS / TAP WATER
Research Abstract

1) DNA strand breaks induced by chlorinated organo compounds containing in tap water
Thirteen chlorinated organo compounds were added to the standard of tap water quality in 1993 in Japan. We assessed the DNA strand breaks in human cells induced by eleven these chlorinated chemicals, using the FADU methods. We estimated DNA strand breaks in human fibroblast cells, W138-VA13. DNA strand breaks were induced by 1,2-dichloroethane and 1,3-dichloropropene.
2) Detection and Quantification of DNA Strand Breaks in Human Cells Induced by Contaminants in Japanese Tap Waters.
We tried to detect DNA strand breaks caused by tap waters. Tap water samples were concentrated using the Sep-pak C_<18> and DNA strand breaks induced in human cells were measured by FADU assay. The tap water samples of Osaka City induced dose dependent increase in DNA strand breaks in human cells. But this DNA toxicity was reduced by rat liver S9 fraction, glutathione and water boiling.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Matsuda: "Molecular analysis of mutations induced by 2-chloroacetaldehyde, the ultimate carcinogonic form of vinyl chbride, in human cells using shuttle vectors" Carcinogenesis. 16 (10). 2389-2394 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松田知成: "水道水中有機塩素化合物のヒト細胞に対するDNA損傷性の評価" 環境変異原研究. 17. 161-167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Matsuda: "Detection and quantification of DNA strand breaks in human cells induced by contaminants in Japanese tap waters" Wat. Sci. Tech.13 (6). 297-304 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsuda: "Molecular analysis of mutations induced by-2-chloroacetaldehyde, the ultimate carcinogenic form of vinyl chloride, in human cells using shuttle vectors" Carcinogenesis. 16-10. 2389-2394 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsuda: "DNA strand breaks in human cells induced by chlorinated organo compounds containing in tap water" Environ.Mut.Res.Commun. 17. 161-167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsuda: "Detection and quantification of DNA strand breaks in human cells induced by contaminants in Japanese tap waters" Wat.Sci.Tech. 33-6. 297-304 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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