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1996 Fiscal Year Final Research Report Summary

Changes in neuroactive substances in a rat model of neuropathic pain and the role of hyperexcitability in neurons of spinal cord-dorsal colum-thalamus pathway

Research Project

Project/Area Number 07457007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionHYOGO COLLEGE OF MEDICINE

Principal Investigator

NOGUCHI Koichi  HYOGO COLLEGE OF MEDICINE,PROFESSOR, 医学部, 教授 (10212127)

Project Period (FY) 1995 – 1996
Keywordsneuropathic pain / spinal dorsal horn / gracile nucleus / Fos / dorsal root ganglion / neuroactive substances
Research Abstract

The aim of this study was to examine the change in mRNA expression of inhibitory amino acid (GABAA and glycine) receptor subunits and neuropeptides in DRG of an experimental rat model of neuropathic pain. Rats received tight ligations of left L5 spinal nerves under deep anesthesia. After confirmation of the presence of neuropathic pain behaviors at 7th postoperative days, L4,5 DRG and spinal cord were dissected out for in situ hybridization and immunohistochemistry. L5 DRG neurons showed the down-regulated expression ofmRNAs of gamma2-GABAA receptor and beta1-glycine receptor subunits. Immunohistochemistry with calcitonin gene-related peptide antibody revealed that increased immunostaining in laminae III-IV of L4-5 spinal cord These data suggest that depressed inhibitory effect of amino acid and increased excitatory effect ofneuropeptide on dorsal horn neurons may contribute to the pathomechanisms of neuropathic pain.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Noguchi K.et al: "Substonce Pinduced by penpleral nerve ingury in primary offevent Sonsory neurons and its effect on dorsal columin nucleus neurons" Journal of Neuroscience. 15. 9633-9643 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kashiba,H. et al: "Co expressin of trk family membors and low‐affinity ncurotrophin receptors in rat dorsal voot ganglion neurons" Molecular Bran Research. 30. 158-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MiKi. K. et al: "CSRP nerease in the rat spinal dusal horn and dorsi/column nucleus following penpheral nerve injury,Up‐repulation" Nearoscience. (in Press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi, K., Kawai, Y., Fukuoka, T., Senba, E.and Miki, K.: "Substance p induced by peripheral nerve injury in primary afferent sensory neurons and its effect on dorsal column nucleus neurons" J.Neurosci.15. 7633-7643 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashiba, H., Noguchi, K., Ueda, Y.and Senba, E.: "Coexpression of trk family members and low-affinity neurotrophin receptors in rat dorsal root ganglion neurons" Mol.Brain Res. 30. 158-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miki, K., Fukuoka, T., Tokunaga, A.and Noguchi, K.: "Calcitonin gene-related peptide increase in the rat spinal dorsal horn and dorsal column nucleus following peripheral nerve injury : Up-regulation in a subpopulation of primary afferent sensory neurons" Neuroscience. (In Press.).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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