1997 Fiscal Year Final Research Report Summary
Search for factors that regulate axial length of eye and for therapy of amblyopia
Project/Area Number |
07557110
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 展開研究 |
Research Field |
Ophthalmology
|
Research Institution | KOBE UNIVERSITY |
Principal Investigator |
YAMAMOTO Misao Kobe University, Dept.of Ophthalmology, Professor, 医学部, 教授 (40182654)
|
Co-Investigator(Kenkyū-buntansha) |
SEKIYA Yoshibumi Kobe University, Dept.of Ophthalmology, 医学部・附属病院, 講師 (20226666)
|
Project Period (FY) |
1995 – 1997
|
Keywords | myopia / chick / axial length / growth factor / nitric oxide synthase / visual cortex |
Research Abstract |
We have measured the refractive error and axial length in infants with high myopia. Visual acuity in bilateral high myopia was lower than that unilateral high myopia. Worse visual prognsis in unilateral high myopia has been attributed to anisometripia and delay in management. We investigated the expression of the PV in the rat visual pathway. Monocular enucleation did not affect the expression of PV in the rat visual cortex. It was suggested that the expression of PV could be regulated by multiple factor. We examined the expression of zif268 and c-fos in visual cortex of young rat. The response of c-fos to photo stimulation are associated with adrenergic neurotoxin in the visual cortex of young rat. We have also investigated the role of plasminogen activator that activates growth factors such as transforming growth factor-B and the role of plasminogen activator inhibitor. Plasminogen activator increased the axial length of control chick eye, while plasminogen activator inhibitor decreased the axial length of experimental myopic chick eye. The data suggested that these mediated the mechanisms that regulating the myopic eye growth. We have also investigated the roles of nitric oxide synthase in experimental myopic chick eye. We demonstrated the expressions of three nitric oxide synthases ; inducible type (iNOS), brain type (bNOS) and endthelial type (eNOS) in chick eye. Expression of iNOS mRNA was highest in the control ocular tissue, followed by bNOS.Expression of eNOS was faint. Form-deprivation reduced the iNOS and bNOS mRNA expressions only iNOS protein showed significant reduction. The iNOS and eNOS protein were found mainly in the outer part of the photoreceptor layr, RPE layr and choroid. bNOS alone was found in the outer nuclear layr. It is suggested that iNOS may be involved in the mechanisms regulating the myopic eye growth.
|
Research Products
(10 results)