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1996 Fiscal Year Final Research Report Summary

Analysis for the mechanism of gastric mucosal damage by Helicobacter pylori infection

Research Project

Project/Area Number 07670322
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyorin University

Principal Investigator

KAMIYA Shigeru  Kyorin UniversitySchool of Medicine, Department of Microbiology, Professor, 医学部, 教授 (10177587)

Co-Investigator(Kenkyū-buntansha) OSAKI Takako  Kyorin University School of Medicine, Division of Flowcytometry.Research associa, 医学部, 助手 (90255406)
YAMAGUCHI Hiroyuki  Kyorin University School of Medicine, Department of Microbiology, Research assoc, 医学部, 助手 (40221650)
Project Period (FY) 1995 – 1996
KeywordsH.pylori / chronic gastritis / peptic ulcer / cytotoxin / cytokine / heat shock protein / adhesin / active oxygen
Research Abstract

Among various virulence factors of Helicobacter pylori, adhesion factor, vacuolating toxin (VT), cytokines (particularly, interleukin-8 (IL-8)), heat shock protein (HSP) and active oxygen were focused to analyze the mechanism of gastric mucasal damage by H.pylori infection.
According to flowcytometric (FCM) analysis, it was shown that H.pylori strains adhere more effectively to gastric epithelial cells than intestinal cells. In addition, H.pylori has hemagglutinating (HA) activity with erythrocytes of guinea pig, rabbit, human, sheep, chicken, horse and bovine. It was also demonstrated that HA activity of H.pylori strains may correlate with adhesion activity to gastric cells. About half of H.pylori strains produce VT.Culture supernates of VT-producing H.pylori strains inhibited secretion of gastric acid by guinea pig parietal cells, but that of VT-non-producing strains not. By analysis of 2nd messengers (Ca^<2+> and cyclic AMP) in signal transduction for secretion of gastric acid, it wa … More s suggested that VT may directly inhibit proton pump H^+-K^+- ATPase. It was shown that H.pylori induces secretion of IL-8 by gastric epithelial cells following its adhesion to the cells. Similarly, it was demonstrated that co-incubation of human peripheral neutrophils with H.pylori resulted in a generation of active oxygen (superoxide). However, neither IL-8 nor active oxygen was induced by culture supernates of H.pylori strains. HSP60 (molecular weight ; 60kDa) was equally detected in 10 H.pylori strains examined in immunoblot analysis. On the other hand, HSP60 on the surface of H.pylori was differently expressed among H.pylori strains in FCM analysis. In addition, the expression rate of HSP60 on the surface correlated with adhesion activity of H.pylori strains. The epitope reactable with monoclonal antibody against H.pylori HSP60 was detected on the surface of gastric epithelial cells, suggesting that HSP60 might be a trigger for chronic inflammation or mucosal damage in gastric tissue.
These results indicate that adhesion factor, VT,cytokines, active oxygen and HSP60 are all closely associated with the occurrence of gastric mucosal damages, and the mechanism for the mucosal damage secms to be multifactorial. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Yamamoto-Osaki T et al: "Adherence of Helicobacter pylori to cultured human gastric carcinoma cells." Eur.J.Gastroenterol.Hepatol.7. 89-92 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi H et al.: "Induction and epitope analysis of heat shock protein of Helicobacter pylori." J.Gastroenterol.31. 12-15 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi H et al.: "The effect of Helicobacter pylori on gastric acid secretion by the isolated parietal cells from a guinea pig." Scand.J.Gastroenterol.31. 428-433 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi H et al.: "Flow cytometric analysis of the heat shock protein 60 expressed on the cell surface of Helicobacter pylori." J.Med.Microbiol.45. 270-277 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osaki T et al.: "Studies on the relationship between adhesive activity and haemagglutination by Helicobacter pylori." J.Med.Microbiol.46. 117-121 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi H et al.: "Heat-shock protein 60 homologue of Helicobacter pylori is associated with adhesion of H.pylori to human gastric epithelial cell." J.Med.Microbiol.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神谷 茂: "ヘリコバクター・ピロリ-最新知見からの報告" 医薬ジャーナル社, 271 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神谷 茂,山口博之: "別冊・医学のあゆみ“生体防御最前線"" 医歯薬出版, 206 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi H,Osaki T,Taguchi H,Hanawa T,Yamamoto T & Kamiya S: "Induction and epitope analysis of heat shock protein of Helicobacter pylori." J.Gastroenterol.31 (Suppl IX). 12-15 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi H,Kamiya S,Suzuki T,Kohda K,Muramatsu S,Kurumada T,Ohta U,Miyazawa M,Kimura N,Mutoh N,Shirai T,TAkagi A,Harasawa S,Tani N & Miwa T: "The effect of Helicobacter pylori on gastric acid secretion by isolated parietal cells from a guinea pig." Scand J Gastroenterol. 31 (4). 428-433 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi H,Osaki T,Taguchi H,Hanawa T,Yamamoto T & Kamiya S: "Flowcytometric analysis of the heat shock protein 60 expressed on the cell surface of Helicobacter pylori." J.Med.Microbiol.45 (4). 270-277 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osaki T,Yamaguchi H,Taguchi H,Kumada J,Ogata S & Kamiya S: "Stidies on relationship between adhesion activity and haemagglutination activity of Helicobacter pylori." J.Med.Microbiol.46 (2). 117-121 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi H,Krihara N,Taguchi H,Hanawa T,Yamamoto & Kamiya S: "Heat-shock protein 60 homologue of Helicobacter pylori is associatid with adhesion of H,pylori to human gastric epithelial cell." J.Med.Microbiol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takagi S,Kamiya S,Koga Y,Ohta U,Kobayashi H,Shirai T,Harasawa S & Miwa T: "Analysis of IL-8 secretion induced by Helicobacter pylori from gastric epithelial cell line (MKN45) : A mechanism independent of cytotoxicity." J.Gastroenterol.Hepatol.(in press). (1997)

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      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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