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1996 Fiscal Year Final Research Report Summary

Analysis of the new nuclear factor mel-18 which affects the development and signaltransduction of B cells

Research Project

Project/Area Number 07670365
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionChiba University, School of Medicine

Principal Investigator

KANNO Masamoto  School of Medicine, Chiba University ; Assoc.Prof., 医学部, 助教授 (40161393)

Project Period (FY) 1995 – 1996
Keywordscell cycle / signal transduction / antigen receptor / Polycomb group gene
Research Abstract

mel-18 is a mammalian homologue of the Drosophila Polycomb group (PcG) gene encoding a sequence-specific transcriptional repressor. We found that the overexpression of mel-18 causes the cell cycle arrest of B-cells after stimulation of the BCR.Molecular analysis revealed that the downregulation of cyclin D2, cyclin E,CDK4, CDK6, CDK7, and CDC25A causes the impaired activities of cyclin-dependent kinases, resulting in hypophosphorylation of the Rb protein. We also demonstrated that c-myc is a direct target of Mel-18 and is downregulated upon mel-18 overexpression. c-Myc is known to regulate positively the expression of cdc25a and cyclin E.Thus we conclude that mel-18 negatively regulates the cell cycle progression through a new cascade starting from mel-18 to c-myc then cdc25a or cyclin E.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Ohki-Hamazaki,H.: "TCR reportoire in early fetal mouse thymus." Int.Immunol.3. 493-499 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanno,M.: "mel-18,a Polycomb group-related mammalian gene,encodes transcriptional negative regulator with tumor suppressive activity." EMBO.J.14. 5672-5678 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsu,O.: "Cloning and characterization of two transcripts generated from the mel-13 gene positioned adjacent to the mammalian Polycomb group-related gene mel-18" Biochem.Biophys.Acta. 1305. 109-112 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akasaka,T.: "A role for mel-18,a Polycomb group-related vertebrate gene,during the anteroposterior specification of the axial skeleton." Development. 122. 1513-1522 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 菅野雅元: "Immunology Frontier「マウスポリコム遺伝子産物の免疫系における機能」" メディカルレビュー社, 10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohki-Hamazaki, H., Makino, Y., Kanno, M., Koseki, H.and Taniguchi, M.: "TCR reportoire in early fetal mouse thymus." Int.Immunol.3. 493-499 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanno, J., Hasegawa, M., Ishida, A.and Taniguchi, M.: "mel-18, a Polycomb group-related mammalian gene, encodes transcriptional negative regulator with tumor suppressive activity." EMBO.J.14. 5672-5678 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsu, O., Kanno, R., Isono, K., Taniguchi, M.and Kanno, M: "Cloning and characterization of two transcripts generated from the mel-13 gene positioned adjacent to the mammalian Polycomb group-related gene mel-18." Biochem.Biophys.Acta. 1305. 109-112 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akasaka, T., Kanno, M., Balling, R., Mieza, M.A., Taniguchi, M.and Koseki, H.: "A role for mel-18, a Polycomb group-related vertebrate gene, during the anteroposterior specification of the axial skeleton." Development. 122. 1513-1522 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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