1996 Fiscal Year Final Research Report Summary
Development of Specific Immunotherapy For Autoimmune Hepatitis-Resaerch On T cell Vaccination
| Project/Area Number |
07670595
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | HIROSHIMA UNIVERSITY |
|---|
Principal Investigator |
NAKANISHI Toshio HIROSHIMA UNIVERSITY Medical Hospital ASSOCIATE PROFESSOR, 医学部・附属病院, 助教授 (20136089)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Keywords | Autoimmune Hepatitis / Experimental Hepatitis / T cell Receptor / alphabeta T cell / Vbeta4 positive T cell / Vbeta-TCR / T cell vaccine / lipopolysaccharide |
|---|
| Research Abstract |
We transferred spleen cells from neonatally thymectomized (NTx) mice or normal mice with hepatitis induced by administration of Propionibacterium. acnes (P.acnes) and LPS to NTx mice or normal mice and examined. Hepatitis were observed in 58% recipient mice only when both donor and recipient were NTx mice. When donor or recipient was normal mice, hepatitis was not observed. Neonatal thymectomy was necessory in donor and recipient mice for transfer of hepatitis. NTx mice showed decreased suppressor function and increased extrathymic autoreactive T cells which play an important role in the multiplication of autoreactive clones as well as the prolongation of hepatitis. By flow cytometric analysis, CD4+, Vbeta4+ T cells were increased in the liver of NTx mice with hepatitis induced by P.acnes and LSP.These findings suggest that hepatitis induced in NTx mice by P.acnes and LPS may occur due to the cell-mediated mechanism and the effector cells were CD4+, Vbeta4+ T cells. When Vbeta4+ T cell-depleted spleen cells of NTx mice with hepatitis by injection of P.acnes and LPS were transferred, transfer of hepatitis was not observed. Vbeta4+ T cell is necessory for transfer of hepatitis. We suggest that experimental hepatitis induced in NTx mice by P.acnes and LPS involved autoimmune mechanism and the effector cells were CD4+, Vbeta4+ T.cells.
|
