1996 Fiscal Year Final Research Report Summary
Recycling of glucose transporter and insulin action
| Project/Area Number |
07671106
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内分泌・代謝学
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| Research Institution | Gunma University |
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Principal Investigator |
SHIBATA Hiroshi Institute for Molecular and Cellular Regulation, Department of Cell Biology, Gunma University, Associate Professor, 生体調節研究所, 助教授 (20235584)
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| Project Period (FY) |
1995 – 1996
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| Keywords | insulin / glucose transporter / GTP-binding protein / Rab4 |
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| Research Abstract |
GTP-binding proteins regulate both exocytotic and endocytotic steps of GLUT4-containing vesicles in rat adipocytes. In the present study, we have shown that a synthetic peptide corresponding to the Rab4 carboxy-terminal domain inhibits insulin-induced GLUT4 translocation, indicating that Rab4 may be essential for the insulin action. Furthemore, insulin stimulated guanine nucleotide exchange on Rab4. This insulin action was markedly attenuated with phosphatidylinositol (Pl) 3-kinase inhibitors, wortmannin or LY294002, suggesting that Pl3-kinase may be an essential signaling component for insulin activation of Rab4.
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