1996 Fiscal Year Final Research Report Summary
Gene therapy-for live cirrhosis by multiplicable hepatocyte and antisense of collagenase-mRNA
| Project/Area Number |
07671353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
OHKOHCHI Nobuhiro School of Medicine, 2nd Department of Surgery, Tohoku University associate professor, 医学部, 講師 (40213673)
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| Co-Investigator(Kenkyū-buntansha) |
DOI Hideyuki School of Medicine, 2nd Department of Surgery, Tohoku University associate profe, 医学部・附属病院, 助手 (90188839)
KATOH Hirotaka School of Medicine, 2nd Department of Surgery, Tohoku University associate profe, 医学部・附属病院, 助手 (00240656)
SHINEHA Ryuzaburo School of Medicine, 2nd Department of Surgery, Tohoku University associate profe, 医学部, 講師 (20192106)
SATOMI Susumu School of Medicine, 2nd Department of Surgery, Tohoku University professor, 医学部, 教授 (00154120)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Liver cirrhosis / Hepatocyte transplantation / Laser device / Gene therapy / Antisense therapy |
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| Research Abstract |
[Purpose] The purpose of this study is to establish a therapy for the liver cirrhosis by a transplantation of proliferative hepatocyte and administration of antisense for mRNA of collagenase. [Materials and Methods] Process of the study were as followed. A) Model of liver cirrhosis is made in mouse and rat and mechanism of fibrosis is analyzed. B) Hybridomas of hepatic cells which can proliferate are made with embrional hepatocytes and parenchymal and nonparenchymal hepatocytes. C) The hybridomas are transplanted into the cirrhotic liver and antisense for the collagenese mRNA is also injected the liver. Conditions of regenerating normal liver are studied. [Results] We made liver cirrhosis using CCl_4 and examined hidroxyprolil activity during changing liver cirrhosis in rats. And we developped a new device for selective cell fusion by Laser device. Using this device we tried to make hybridoma of parenchymal hepatocyte and embrional hepatocyte, which can proliferate. We prepared HVJ liposome using Sendai virus and phospholipid for transfection of antisense of collagenase-mRNA.Studies of investigation in analysis of fibrosing process of liver were still remained.
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