1996 Fiscal Year Final Research Report Summary

Selective Cell Fusion Method by LASER Device

Research Project

Project/Area Number	07671356
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Digestive surgery
Research Institution	Tohoku University
Principal Investigator	KATOH Hirotaka Tohoku University, Second Department of Surgery, School of Medicine, assistant professor, 医学部・附属病院, 助手 (00240656)
Co-Investigator(Kenkyū-buntansha)	OHKOHCHI Nobuhiro Tohoku University, Second Department of Surgery, School of Medicine, assistant p, 医学部, 講師 (40213673) DOI Hideyuki Tohoku University, Second Department of Surgery, School of Medicine, assistant p, 医学部・附属病院, 助手 (90188839) SATOH Shunichi Tohoku University, Department of Technology, associate professor, 素材工学研究所, 助教授 (30162431)
Project Period (FY)	1995 – 1996
Keywords	micro-processing / cell fusion / myeloma cells (SP2) / Ouchterlony method / hybridoma
Research Abstract	We developed a new cell fusion method which was sterile, noncontact and selective technique under the microscope, using the micro-processing device by LASER.By this technique, we succeeded in fusing myeloma cells (SP2) and lymphocyte in mouse. We also defined the proliferation of the fused cells in HAT medium and the function of the fused cells in the Ouchterlony method, i.e. production of IgG.This method is applicable to fuse cells which are difficult to be fused by conventional methods.