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1997 Fiscal Year Final Research Report Summary

Study on the hypoxic glucose output in primary cultured rat hepatocytes

Research Project

Project/Area Number 07671664
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MORIWAKI Katsuyuki  Hiroshima University, Medical Hospital Assistant Professor, 医学部・附属病院, 講師 (30157937)

Co-Investigator(Kenkyū-buntansha) SHIROYAMA Kazuhisa  Hiroshima University, Medical Hospital, Research Associate, 医学部・附属病院, 助手 (30284201)
TANAKA Hiroyuki  Hiroshima Univeristy, Medical Hospital, Research Associate, 医学部・附属病院, 助手 (10274086)
YUGE Osafumi  Hiroshima University, School of Medicine Professor, 医学部, 教授 (40034128)
Project Period (FY) 1995 – 1997
KeywordsPrimary cultured rat hepatocytes / Hypoxia / Glucose Metabolism / Lactate Metabolism / Calcium / Dopamine / Volatile Anesthetics
Research Abstract

Using primary cultured rat hepatocytes, we obtained following results :
1. Fundamental studies on the nature of hypoxic glucose output :
Lactate output was also increased when thehypoxic glucose output increased in the cultured hepatocytes. On the other hand, catecholamines (adrenaline, phenylephrine, isoproterenol) decreased lactate release from the hepatocytes, which might indicate increased uptake of lactate to the hepatocytes. The glucose output from the hepatocytes induced by phenylephrine or isoproterenol was enhanced additively by the concomitant exposure to hypoxia. In addition, hypoxic glucose release was alleviated by exchanging the medium containing calcium to one which contains no calciuim (chileted with EDTA). This finding may suggest hypoxic glucose output is dependent on the inflow of calcium to the hepatocytes to the hepatocytes.
2. Studies on the hypoxic injury of the hepatocytes :
Histological findings : Prolonged hypoxia caused condensation of nuclei and disappearance of the nucleoles, the morphological change of which was positively correlated with leakage of lactate dehydrogenase from the hepatocytes. Glycogen-rich hepatocytes tolerateted well in the hypoxic condition, while glycogen-poor hepatocytes were labile to hypoxia.
3. Pharmacological studies on the effects of agents on the glucose and lactate output under aerobic condition : Volatile anesthetics including halothane, isoflurane and sevoflurane enhanced glucose and lactate output from the hepatocytes in the dose-dependent manner. High dose of dopamine enhanced glucose release from the hepatocytes via activation of beta-receptors.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Moriwaki K.: "Hypoxie glucose and lactate outputs from the liver." Shock. 4. 30 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 城山 和久: "細胞外カルシウムが低酸素による肝細胞からのグルコース放出に及ぼす影響" J.Anesthesia. 9(Supple.). 230 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 城山 和久: "アドレナリン作動薬による肝細胞からのグルコース放出に与える低酸素の影響" J.Anesthesia. 11(Suppl.). 365 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 楠 真二: "低酸素による肝細胞の形態学的変化-ラット初代培養細胞における検討-" J.Anesthesia. 12(Suppl). 381 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 城山 和久: "肝細胞中のグリコーゲン含有量が低酸素による肝細胞障害の程度に及ぼす影響" J.Anesthesia. 12(Suppl.). 381 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuyuki MORIWAKI: "Hypoxic glucose and lactate outputs from the liver -Clinical and experimental evidences." Shock. 4 (Suppl). 30 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuhisa SHIROYAMA: "The effect of hypoxia on the glucose release by adrenergic agonists." Journal of Anesthesia. 9 (Suppl). 230 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuhisa SHIROYAMA: "The effect of extracellular calcium on hypoxic glucose output from the primary cultured rat hepatocytes" Journal of Anesthesia. 9 (Suppl). 230 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinji KUSUNOKI: "Morphological changes induced by hypoxia in primary cultured rat hepatocytes" Journal of Anesthesia. 12 (Suppl). 381 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuhisa SHIROYAMA: "The influence of glycogen content on hypoxic hepatocellular injury" Journal of Anesthesia. 12 (Suppl). 381 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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