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1997 Fiscal Year Final Research Report Summary

The study of inflammatory and defensive mechanisms in otitis media with effusion.

Research Project

Project/Area Number 07671872
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionSapporo Medical University

Principal Investigator

HIMI Tetsuo  Sapporo Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (90181114)

Co-Investigator(Kenkyū-buntansha) HARABUCHI Yasuaki  Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (80208686)
Project Period (FY) 1995 – 1997
KeywordsOtitis media / Cytokine / Chemokine / Neutrophil / Adhesion molecules
Research Abstract

In this project, in vivo induction and regulation of chemokine, cytokines and cell adhsion molecules in the middle ear were investigated using topical inoculation with KLH,lopopolysaccharide (LPS) and Lipoteichoic acid (LTA) in rat model. The expressions of mRNA in the middle ear mucosa was also examined.
At first, we examined the expression of cell adhesion molecules (CAM) in immune-mediated otitis media in the rat, as well as the regulation of these CAM in peripheral blood polymorphonuclear leukocytes (PMN) and lymphocytes upon exposure to middle ear effusion (MEE). The expression of CAM was important for the initiation of otitis media. Moreover, it ws thought that the interaction between the infiltrated PMN and MEE may modify the CAM's expression during the inflammatory process in the middle ear cavity.
Chemokine possesses chemotactic-activating properties toward neutrophils, and may contribute to the pathogenesis of middle ear inflammations. We investigated the production of chemokine in middle ear lavage and that gene expression in the middle mucosa using topical inoculation with LPS and LTA in the rat in vivo model. In contrtast to LPS as gram-negative endotoxin, lipoteichoic acid (LTA) is present within this layr of LPS as gram-negative endotoxin, lipoteichoic acid (LTA) is present within this layr of mo gram-positive bacteria. LTA has been shown to play a sigificant role in the initiation and progression of bacterial infection. Chemokine in middle ear lavage showed time-and dose-dependent production under LPS or LTA stimulation. At the time of peak production, the expression of cytokines and chemokine mRNA,evaluated using polymerase chain reaction, was considerable in the middle ear mucosa.
This investigation of the characteristics of proinflammatory cytokines in the middle ear cavity using rat in vivo model has extended the functional concept of cytokines at the initial stage otitis media.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kamimura, Himi etal: "Cell adhesion molecules in experimental otitismedia in the rat" Acta Otolaryngol(stockh). 116. 857-862 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Himi, Yoshioka etal: "Production and expression of cytokine GRO/CINC-1 in rat nasal mucosa" Acta Otolaryngol(stockh). 117. 123-127 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Himi, Yoshioka etal: "Influence of age in production and expression of GRO/CINC-1 in rat nasal mucosa" Eur Arch otolaryngol. 254. 101-104 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshioka, Himi etal: "In vio induction and regulation of IL-8 like chemokine in rat middleear" Acta otolaryngol(stockh). 117. 724-727 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamimura, Himi: "Regulation of neutrophil migration by LFA-1 and Mac-1 in otitismedia" Eur.Arch.otolaryngol. 254. 150-152 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Himi, Kaireno etal: "Expression profile of VCAM-1(CD106)in the middle ear using radiolabeled m〓" Eur.Arch.otolaryngol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamimura M,Himi T,Kataura A: "Cell adhesion molecules in experimental otitis media in the rat." Acta Otolaryngol (Stockh). 116. 857-862 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamimura M,Himi T,Kataura A.: "Regulation of Neutrophil Migration by LFA-1 and Mac-1 in Otitis Media." Eur Arch Otolaryngol. 254. 150-152 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Himi T,Yoshioka I,Kataura A: "Influence of age in production and expression of GRO/CINC-1 in rat nasal mucosa." Eur Arch Otolaryngol. 254. 101-104 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Himi T,Yoshioka I,Kataura A: "Production and gene expression of IL-8-like cytokine GRO/CINC-1 in rat nasal mucosa" Acta Otolayngol (Stockh). 117. 123-127 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshioka I,Himi T,Kataura A: "In vivo induction and regulation of interleukin-8-like chemokine GRO/CINC-1 in rat middle ear." Acta Otolaryngol (Stockh). 117. 719-723 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshioka I,Himi T: "Production and regulation of inflammatory cytokines in the middle ear" Sapporo Medical J. 66. 79-87 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Himi T,Kairemo KJA,Ramsay HA: "Expression profile of vascular cell adhesion molecule-1 (CD106) in the middle ear using radiolabeled monoclonal antibody" Eur Arch Otolaryngol. in press.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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