1996 Fiscal Year Final Research Report Summary
Analysis of HLA-B27, investigating of the susceptibility of AS and AAU
| Project/Area Number |
07671903
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Saitama Medical School (1996)
The University of Tokyo (1995) |
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Principal Investigator |
JOKO Satoru Saitama Medical School, Faculty of Medicine, Assistant, 医学部, 助手 (80246470)
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| Co-Investigator(Kenkyū-buntansha) |
FUJINO Yujiro Tokyo University, Faculty of Medicine, Lecturer, 医学部, 講師 (30143465)
NUMAGA Jiro Tokyo University, Faculty of Medicine, Lecturer (30189352)
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| Project Period (FY) |
1995 – 1996
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| Keywords | HLA-B27 / Ankylosing Spondylitis / Acute anterior uveitis / Haplotype |
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| Research Abstract |
The strong association between Ankylosing Spondylitis (AS) and HLA-B27 has been reported from many countries, but, very rare from Japan, because AS as well as the frequency of HLA-B27 is rare in Japan as compared with Caucasians. HLA-B27 is also known to be associated with acute anterior uveitis (AAU) which is often complicated with AS.We investigated 46 Japanese patients with AS and 59 with AAU ; 24 patients with AS and AAU,a total number of 81 patients, of which 44 patients carried HLA-B27. PCR-SSCP was employed for B27 DNA subtyping, and the amino acid sequence was confirmed by automated sequencer. We also performed class II DNA typing using PCR-SSCP and-RFLP method. We estimated the linkage disequilibrium between each subtype of HLA-B27 and class II alleles (DR,DQ locus). We found two subtypes of HLA-B27, B^<**>2704 and B^<**>2705. The former was found in 34 (77.3%) patients and the latter in 10 (22.7%). We could not find any differences in the frequencies of 2 HLA-B27 subtypes among AS patients, AAU patients and B27 positive controls. Then AS patients were divided into two groups ; with AAU and without AAU.The frequencies of B27 subtypes were almost equal between the two groups, however, we found a significant difference in the frequency of HLA-DR8, which was increased in the patients with AAU (p<0.0005). This result may suggest that the susceptibility of AAU in the AS patients is influenced by HLA-DR8. HLA-B27 containing HLA-A,B,DR haplotypes frequencies are now being investigated.
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