1996 Fiscal Year Final Research Report Summary
MATRIX METALLOPROTEINASE IN INFECTIOUS KERATITIS
| Project/Area Number |
07671927
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | KUMAMOTO UNIVERSITY |
|---|
Principal Investigator |
MIYAGAWA Shin-ichi KUMAMOTO UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 助手 (10260738)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Koki KUMAMOTO UNIVERSITY SCHOOL OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (70173896)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Keywords | Matrix metalloproteinase / Pseudomonal virulence factor / Rabbit keratocyte |
|---|
| Research Abstract |
Purpose : Pseudomonal keratitis often results in severe corneal ulcer and perforation with poor visual prognosis. The corneal lesions could be attributable to proteolytic enzymes including MMP released in the corneas during the infection. However, little is known about the role and behavior of MMP in pseudomonal keratitis. Therefore, we investigated the effects of pseudomonal virulence factors on the cultured keratocyte with emphasis on MMP release. Methods : After rabbit keratocytes reached to confluent growth in PRMI-1640 containing 1o% FCS,the medium was changed to RPMI-1640 without FCS containing one of the following pseudomonal virulence factors : elastase (100,30,10,3 ng/ml), alkaline protease (100,30,10,3 ng/ml), exotoxin A (10,3,1,0.3 mg/ml) and LPS (30,10,3,1 mg/ml). At 48 hours, the culture media were harvested and processed for gelatin and casein zymography to analyze proteolytic enzymes (MMP). Morphologic changes of keratocytes under these conditions were also investigated. Results : Gelatinase A (MMP-2) was detected slightly in the control medium. Pseudomonal elastase enhanced remarkably the release of both MMP-2 and MMP-9 from the keratocytes. The activated forms of these MMPs were also observed. Alkaline protease showed a similar effect but did not activate any MMPs. Similar enhancements were observed also with LPS and to a lesser extent with exotoxin A.Conclusions : From the results that pseudomonal virulence factors enhanced the release of MMP from keratocytes, it is reasonable to conclude that corneal MMP might also contribute to ulceration in pseudomonal keratitis.
|
