1996 Fiscal Year Final Research Report Summary
RECORDING AND ANALYSES FOR AUTHENTIC INPUTS FROM THE ENTOPEDUNCULAR NUCLERU TO THE THALALMUS
Project/Area Number |
07680907
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
神経・脳内生理学
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Research Institution | The Institute of Physical and Chemical Research (RIKEN) |
Principal Investigator |
FUTAMI Takahiro The Institute of Physical and Chemical Research (RIKEN), アルゴリズム研究チーム, フロンティア研究員 (40209143)
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Project Period (FY) |
1995 – 1996
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Keywords | entopeduncular nucleus / subthalatmus / VA-VL of thalamus / intracellular recording / labeling with PHA-L / jxtacellular iontophoretic labeling with biocytin / axonal projection pattern |
Research Abstract |
The synaptic inputs from the entopeduncular nucleus (ENT) to thalamocortical neurons (TCNs) in the ventroanterior-ventrolateral complex (VA-VL) of the thalamus were investigated. Effects of stimulation of the ENT and the brachium conjunctivum (BC) were examined by recording intracellular potentials from VA-VL neurons in anesthetized cats. TCNs were identified by their antidromic responses to stimlation of the pericruciate cortex. Stimulation of the ENT evoked monosynaptic IPSPs in TCNs, while stimulation of the BC evoked monosynaptic EPSPs followed bgy disynaptic IPSPs. Only IPSPs were evoked in virtually all of the TCNs receiving ENT input. Mapping experiments revealed that TCNs in the rostrodorsal part of the VA-VL predominantly received input from the ENT,while TCNs in the more caudoventral part of the VA-VL received input from the BC.Less than 10% of the TCNs examined received convergent inputs from both the ENT and BC and those cells were located at the border between the ENT-and BC-receiving areas of the the VA-VL.TCNs receiving ENT input projected to area 6, while TCNs receiving BC input projected to areas 4 and 6. A single cell had multiple axons and simultaneously innervated areas other than the thlamus in juxtacellular iontophoretic labeling with biocytin. These results suggest that the outputs from the cerebellum and the basel ganglia are conveyed to the motor cortex via different channels in the motor thalamus Stimlation of the subthalamus (STh) evoked monosynaptic and disynaptic IPSPs in TCNs. The disynaptic IPSPs showed a temporal and a spatial facilittion by sing a conditioning and test paradigm between STh and ENT.These results suggested that the STh inputs toTCNs were evoked via ENT ecls. The STh induced monosynaptic IPSPs might be evoked by axon reflexes.
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Research Products
(12 results)