The development of inflammatory models with endotoxin and its evaluation

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07807209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 応用薬理学・医療系薬学
• Research Institution: Kitasato University School of Pharmaceutical Sciences
• Principal Investigator: UENO Akinori Kitasato University School of Pharmaceutical Sciences, Department of Pharmacology, Associate Professor, 薬学部, 助教授 (00112657)
• Co-Investigator(Kenkyū-buntansha): UTSUNOMIYA Iku Kitasato University School of Pharmaceutical Sciences, Department of Pharmacolog, 薬学部, 助手 (70168722) ; HAYASHI Izumi Kitasato University School of Pharmaceutical Sciences, Department of Pharmacolog, 薬学部, 講師 (90172999)
• Project Period (FY): 1995 – 1996
• Keywords: endotoxin / bradykinin / des-Arg9-bradykinin / receptor / induction / inflammation / cyclooxygenase / rats

Research Abstract

It was shown that bradykinin might involve in the permeability increase with intradermally injected endotoxin in rats. It was found that the hypotensive and contractile responses to des-Arg9-bradykinin were induced in rats previously treated with endotoxin. From the experimental results with antagonists and inhibitors of protein synthesis, it could be concluded that both responses mediated via newly induced B1 receptors. Furthermore, cyclooxygenase-2 was also induced by treatment of rat macrophages with endotoxin. And the inflammatory models was established by the intraperitoneal or intrapleural injection of endotoxin into rats.

Research Products

• [Publications] Ueno,A.,et al: "Involvement of bradykinin in endotoxin-induced vascular permeability increase in the skin of rats." Eur,J.Pharmacol.284. 211-214 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tokumasu T.,et al: "A hypotensive response induced by des-Arg9-bradykinin in young Brown/Norway rats pretreated with endotoxin." Eur,J.Pharmacol. 274. 225-228 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ueno,A.,et al: "The mediators involved in endotoxin-induced vescular permeability increase in the rat and their interactions." Jap.J.Pharmacol. 70. 285-290 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsumoto,H.,et al: "Concordant induction of prostaglandin E2 synthase with cyclooxygenase-2 leads to preferred production of prostaglandin E2 over thrombaxane and prostaglandin D2." Biochem,Biophys,Res,Comn.,. 230. 110-114 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ueno, A., Tokumasu, t., Naraba, H.and Oh-ishi, S.: "Involvement of bradykinin in endotoxin-induced vascular permeability increase in the skin of rats." Eur.J.Pharmacol.284. 211-214 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tokumasu, T., Ueno, A.and Oh-ishi, S.: "A hypotensive response induced by des-Arg9-bradykinin in young Brown/Norway rats pretreated with endotoxin." Eur.J.Pharmacl.274. 225-228 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ueno, A.Tokumasu, T., Naraba, H.and Oh-ishi, S.: "The mediators involved in endotoxin-induced vascular permeability increase in the rat and their interactions." Jpn.J.Pharmacol.70. 285-290 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsumoto, H., Naraba, H., Murakami, M., Kudo, I., Yamaki, K., Ueno, A.and Oh-ichi, S.: "Concordant induction of prostaglandin E2 synthase with cyclooxygenase-2 leads to preferred production of prostaglandin E2 over thrombaxane and prostaglandin D2 in lipopolysaccharide-stimulated rat peritoneal macrophages." Biochem.Biophys.Res.Comn.230. 110-114 (1997)
  • Description: 「研究成果報告書概要(欧文)」より