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1997 Fiscal Year Final Research Report Summary

ANALYSIS OF GENES EXPRESSED IN PREIMPLANTATION MOUSE EMBRYOS.

Research Project

Project/Area Number 08044221
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Developmental biology
Research InstitutionSCHOOL OF MEDICINE,TOKAI UNIVERSITY

Principal Investigator

KIMURA Minoru  TOKAI UNIVERSITY,SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (10146706)

Co-Investigator(Kenkyū-buntansha) SAKURAIN Takayuki  TOKAI UNIVERSITY,SCHOOL OF MEDICINE,POST DOCTORAL FELLOW, 医学部, 奨励研究員
HWANG S.Y.  The Jackson Laboratory, Research A
OH Bermseok  THE JACKSON LABORATORY,RESEARCH ASSOCIATE, Research A
KNOWLES B.B.  THE JACKSON LABORATORY,PROFESSOR, Professor
SOLTER Davor  MAX-PLANCK-INSTITUT FUR IMMUNOBIOLOGIE,DIREKTOR, Direktor
HWANG Sue Yun  THE JACKSON LABORATORY,RESEARCH ASSOCIATE
Project Period (FY) 1996 – 1997
Keywordsmouse / early embryo / gene expression / cDNA / polyA / SSEC-D / polyA addition / maternal RNA
Research Abstract

In the past two years, the fund from this grant helps us greatly to interact with many excellent researchers in Europe and in the US,and organize very fruitful collaboration with them.
The long-term goal of the project was the understanding of regulation of gene expression in early mammalian embryos. One of the main obstacles in this field had been the difficulty in obtaining large quantity of experimental materials, and thus molecular understanding of the precess had been very limited.
In order to circumvent this obstacle, we decided to begin the collaborative research by isolating genes that are expressed in mouse early embryos. With various "state-of-art" genetic engineering techniques, we were able to construct cDNA libraries from small amounts of mouse early embryos. We then established the whole-mount in situ hybridization technique, and analyzed expression patterns of our several novel cDNAs in early embryos as well as in various mouse tissues.
Among many valuable outcomes of this … More collaboration, by far the most important findings came from the analyzes of one of the cDNA clones, called SSEC-D.Analyzes of the SSEC-D transcripts in early mouse embryos have revealed that the maternally inherited SSEC-D RNA species become significantly elongated in a stepwise fashion during the 1-2 cell embryonic stage, and then become shortened and degraded by the late 2-cell stage. The chain-length elongation of the SSEC-D RNA appears to be due to addition of extra poly A sequences (300-400 nucleotides) to the 3'-end of RNA.It is particularly intriguing that this dynamic changes of the SSEC-D transcripts are independent of (RPase II-directed) zygotic transcription, DNA replication, or even fertilization. It thus appears that the changes of the SSEC-D RNA reflect an autonomous maternal program, which continues to play roles even after fertilization. We are currently preparing to report our novel findings, and plan to extend further the analyzes to understand the biological causes and effects of this phenomenon. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Miyado,K.,Kimura,M.et al.: "Differencial Expression of mRNAs for M31 and M32,Murine Homologues of Drosophilla Heterochromatin Protein 1(HP1),During Murine Embryogenesis." Biochemistry and Molecular Biology International. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chen,L.,Kimura,M.et al.: "Molecular cloning of a leucine zipper motif-containing novel cDNA specifically expressed in adult mouse testis." DNA Sequence. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chen,I.,Kimura,M.et al.: "Molecular cloning and analysis of novel cDNAs spscifically expressed in adult mouse testis.." Biochem.Biophys.Res.Comm.240. 261-268 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura,M.,Sato,M.et al.: "Overexpression of a Minor Component of Myelin Basic Protein Isoform(17.2kDa)can Restore Myelinogenesis in Transgenic Shiverer Mice." Brain Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kikuti,Y-Y.,Kimura,M.et al.: "Physical Mapping 220 kb Centromeric of the Human MHC and DNA Sequence Analysis of the 43kb Segment Including the RING,HKE6 and HKE4 Genes." Genomics. 42. 422-435 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mizuki,N.,Kimura,M.et al.: "Triplet Repeat Polymorphism in the Transmembrane Region of the MICA Gene;A Strong Association of Six GCT Repetitions with Behcet's Disease." Proc.Natl.Acad.Sci.94. 1298-1303 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyado, K., Kimura, M.et al.: "Differential Expression of mRNAs for M31 and M32, Murine Homologues of Drosophilla Heterrochromatin Protein (HP1), During Murine Embryogenesis." Biochemistry and Molecular Biology International. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chen l., Kimura, M.et al.: "Molecular cloning of a leucine zipper motif-containing novel cDNA specifically expressed in adult mouse testis." DNA Sequence. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chen L., Kimura, M.et al.: "Molecular cloning and analysis of novel cDNA specifically expressed in adult mouse testis." Biochem.Biophys.Res.Comm.240. 261-268 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, M., Sato M.et al.: "Overexpression of a Minor Component of Myelin Basic Protein Isoform (17.2kDa) can Restore Myelinogenesis in Transgenic Shiverer Mice." Grain Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kikuti, Y-Y., Kimura, M.et al.: "Physical Mapping 220kb Centromeric of the Human MHC and DNA Sequence Analysis of the 43kb Segment including the RING,HKE6 and HKE4 Genes." Genomics. 42. 422-435 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizuki, N., Kimura, M.et al.: "Triplet Repeat Polymorphism in the Transmembrane Region of the MICA Gene ; A Strong Association of Six GCT Repetitions with Behcet's Disease." Proc.Natl.Acad.Sci.94. 1298-1303 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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