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1997 Fiscal Year Final Research Report Summary

Molecular analysis on osteoporosis using transgenic mice

Research Project

Project/Area Number 08044258
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Orthopaedic surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

NODA Masaki  Tokyo Medical and Dental University Medical Research Institute, Professor, 難治疾患研究所, 教授 (50231725)

Co-Investigator(Kenkyū-buntansha) DENHARDT Dav  ラトガース大学, 生化学研究所, 所長、教授
KAWAGUCHI Nanako  Tokyo Medical and Dental University Medical Research Institute, Assistant Profes, 難治疾患研究所, 助手 (10200700)
TAMURA Masato  Kagoshima Univ., School of Dent., Assistant Professor, 歯学部, 助手 (30236757)
NIFUJI Akira  Tokyo Medical and Dental University Medical Research Institute, Lecturer, 難治疾患研究所, 講師 (00240747)
DENHARDT David T.  Rutgers Univ., Dept.of Biological Science, Professor
Project Period (FY) 1996 – 1997
Keywordsosteoporosis / osteoblast / osteoclast / carvaria / ascorbic acid / knock out animal / tartrate resistant acid phosphatase / osteopontin
Research Abstract

Murine osteopontin gene knock out mice were produced and the phenotypes were analyzed. Major axons were included in the region which was replaced with an insertion cassette sequence. ES cells obtained by the homologous recombination were introduced into blastocysts. Mice were born and were mated to generate knock out mice. Tibiae and femora were taken out of the knock out mice and were analyzed for their morphology. Similar features have been found in control and knock out mice in most of the gross skeletal architecture and histology. However, in vitro osteoclastogenesis monitored by examining tartrate resistant acid phosphatase was found to be different. Also calvarial osteoblastic cells were also shown to be normal to form bone in the presence of ascorbic acid. These mice show the importance of osteopontin in bone metabolism.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] T.Yamashita,M.Noda et al.: "Elongation of the epiphyseal trabecular bone associated with precocious aging in transgenic mice carrying an insertional mutaiton in Klotho gene locus." Journal of Bone and Mineral Research. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tsuji,M.Noda et al.: "Perturbation of BMP signaling in somitogenesis resulted in vertebral and rib malformation in the axial skeletal formation." Bone. 22・2. 87-92 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Murakami,M.Noda et al.: "Expression of Indiana Hedgehog in Osteoblasts and Its Posttranscriptional Regulation by TGFβ" Endoclinology. 138・5. 1972-1978 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Matsue,M.Noda et al.: "Helix-Loop-Helix Type Trascription Factor,HES-1, Is Expressed In Osteoblastic Cells, Suppressed By 1,25(OH)_2D_3 And Modulates 1,25(OH)_2D_3Enhancement of Osteopontin Expression" Bone. 20・4. 329-334 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Liu、M.Noda et al.: "Overexpression of a single helix-loop-helix type transcription factor, scleraxis,enhances aggrecan gene expression in osteoblastic osteosarcoma ROS17/2.8 cells." Journal of Biological Chemistry. 272・47. 29880-29885 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Liu、M.Noda et al.: "Scleraxis is expressed in C2C12 myoblasts and its level is down-regulated by bone morphogenetic protein-2 (BMP2)." Journal of Cellular Biochemistry. 67. 1-9 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ezura, M.Noda et al.: "Identification of a novel suppressive vitamin D response sequence in the 5'flanking region of murine ld1 gene." Journal of Biological Chemistry. 272・47. 29865-29872 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Nifuji,M.Noda et al.: "Perturbation of BMP signaling in somitogenesis resulted in vertebral and rib malformation in the axial skeletal formation." Journal of Bone and Mineral Research. 12・3. 332-342 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kawaguchi, M.Noda et al.: "Messenger RNA Expression of the Genes Encoding Receptors for Bone Morphogenetic Protein (BMP) and Transforming Growth Factor-β(TGF-β) in the Cells from the Posterior Longitudinal Ligament in Cervical Spine" Endocrine. 5・3. 307-314 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Liu、M.Noda et al: "Sclerotome-related helix-loop-helix type transcription factor (scleraxis) mRNA is expressed in osteoblasts and its level is enhanced by type-β transforming growth factor" Journal of Endocrinology. 151. 491-499 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yamashita, A.Nifuji, K.Furuya, Y.Nabeshima and M.Noda: "Elongation of the epiphyseal trabecular bone associated with precocious aging in transgenic mice carrying an insertional mutation in Klotho gene locus." Journal of Bone and Mineral Research. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tsuji, Y.Ito and M.Noda: "Perturbation of BMP signaling in somitogenesis resulted in vertebral and rib malformation in the axial sketetal formation." Bone. 22-2. 87-92 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Murakami, A.Nifuji and M.Noda: "Expression of Indiana Hedgehog in Osteoblasts and its Posttranscriptional Regulation by TGFbeta." Endocrinology. 138-5. 1972-1978 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Matsue, R.Kageyama, D.T.Denhardt and M.Noda: "Helix-Loop-Helix Type Transcription Factor, HES-1, Is Expressed In Osteoblastic Cells, Suppressed By 1.25 (OH)_2D_3 And Modulates 1.25 (OH)_2D_3 Enhancement of Osteopontin Expression." Bone. 20,10-4. 329-334 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Liu, H.Watanabe, Y.Yamada, E.Olson, A.Nifuji and M.Noda: "Overexpression of single helix-loop-helix type transcription factor, scleraxis, enhances aggrecan gene expression in osteoblastic osteosarcoma ROS17/2.8 cells." Journal of Biological Chemistry. 272-47. 29880-29885 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Liu, J.Wozney, A.Nifuji, E.Olson and M.Noda: "Scleraxis is expressed in C2C12 myoblasts and its level is down-regulated by bone morphogenetic protein-2 (BMP2)." Journal of Cellular Biochemistry. 67. 1-9 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ezura, O.Tourmay A.Nifuji and M.Noda: "Identification of a novel suppressive vitamin D response sequence in the 5' flanking region of murine ld1 gene" Journal of Biological Chemistry. 272-47. 29865-29872 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Nifuji, O.Kellerman, Y.Kuboki, J.Wozney and M.Noda: "Perturbation of BMP signaling in somitogenesis resulted in vertebral and rib malformation in the axial skeletal formation." Journal of Bone and Mineral Research. 12-3. 332-342 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kawa-uchi, K.Furuya, K.Shinomiya, I.Yama-ura, Y.Kurosa, J.M.Wozney, N.Ueno and M.Noda: "Messenger RNA Expression of the Genes Encoding Receptors for Bone Morphogenetic Protein (BMP) and Transforming Growth factor-beta (TGF-beta) in the Cells from the Posterior Longitudinal Ligament in Cervical Spine." Endocrine. 5. 307-314 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Liu, P.Cserjesi, A.Nifuji, E.N.Olson and M.Noda: "Sclerotome-related helix-loop-helix type transcription factor (scleraxis) mRNA is expressed in osteoblasts and its level is enhanced by type-beta transforming growth factor." Journal of Endocrinology. 151. 491-499 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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