1997 Fiscal Year Annual Research Report

細胞増殖因子、形態形成因子の細胞分化における役割

Research Project

Project/Area Number	08045066
Research Institution	The University of Tokushima
Principal Investigator	荒瀬誠治徳島大学, 医学部, 教授 (90108887)
Co-Investigator(Kenkyū-buntansha)	FRANCE J.T. オークランド大学, 医学部, 準教授 CROSIER K.E. オークランド大学, 医学部, 準教授 MERRILEES M. オークランド大学, 医学部, 副学長小阪昌明徳島大学, 医学部, 講師 (10093858) 板倉光夫徳島大学, 医学部, 教授 (60134227)
Keywords	糖尿病 / TGF-β / トランスゼニックマウス / amylin / activin / 同種抹消血移植 / 毛乳頭特異的遺伝子 / 動脈粥状変成 / gap junction
Research Abstract	板倉は、インスリン依存性糖尿病(IDDM)マウスの膵ラ島A細胞に活性型TGF-β1を発現するトランスジェニックマウス(Tg)を作成し、NODマウスの糖尿病の発症が抑制されることを見出した。CooperはAmylinと糖尿病の関係をTgやノックアウトマウスで検討した。小坂は骨髄造血環境での単球、骨髄間質線維芽細胞のActivin-A(Act-A)、follistatin(FS)の産生が、炎症性サイトカインや活性化T細胞の接着を介する細胞間相互作用により調節されることを見出し、Franceは胎盤由来Act-A、CRHの量比が分娩の引き金となることを証明、血清中の両者の量のモニターで、早期分娩の危険性を察知できるとした。河野は小児の自家末梢血幹細胞移植術法(PBSCT)では、PBSCT後のG-CSF投与は必要ないことを見出した。また小児健常人ドナーから末梢血幹細胞(PBSC)を採取する同種移植術も確立した。Crosierらは新型受容体チロシンキナーゼ(Dtk)遺伝子のcDNA、遺伝子座、ligand、機能を検討し、Dtkは胎児期の造血臓器、神経の発生・発育にも必須であることを見出した荒瀬は28個のヒト毛乳頭特異的新型遺伝子を見出し、そのうち10個の新規遺伝子のDNA配列を決定、遺伝子産物機能や皮膚での局在を解析中でノックアウトマウスも作成中である。Greenは、ヒドラ、蛙などの胎児組織にgapjunctionのantisense deoxyoligonucleotide作用させると臓器の形成が妨げられることより、臓器の形成・発育にはGap Junctionを通じての細胞間情報交換が必須であることを見出した。Merrileesは、動脈壁でのTGF-β1とmatrix proteoglycan(Pgとくにversican、biglycan、decorin)の量と分布が粥状変成の危険度が類推できることを見出した。

Research Products

(42 results)

All Other

All Publications (42 results)

[Publications] Takiwaki H, Arase S et al: "Computor simulation of skin color based on a multilayered skin model" Skin Res Technol. 3. 36-41 (1997)
[Publications] Takiwaki H, Arase S et al: "A simple method to estimate CIE-L l.a.b.values of the skin from its videomicroscopic image" Skin Res Technol. 3. 42-44 (1997)
[Publications] Ninomiya Y, Arase S et al: "P53 gene mutation analysis in porokeratosis and porokeratosis-associated squamous cell carcinoma" J Dermatol. 14. 172-178 (1997)
[Publications] Kubo Y, Arase S et al: "Mutations of the INK4a locus in squamous cell carcinomas of human skin" BBRC. 232. 38-41 (1997)
[Publications] Shikiji T, Arase S et al: "A case of episodic angioedema associated with eosinophilia" J Med Invest. 44. 103-108 (1997)
[Publications] Miyamoto H, Arase S et al: "Color analysis of nevus of Ota for evaluation of treatment with a Q-switched alexandrite laser." Skin Res Technol. 3. 45-48 (1997)
[Publications] Miyaoka Y, Arase S et al: "A case of dermatomyositis complicated by thrombotic thrombocytepenic purpura" Dermatology. 194. 68-71 (1997)
[Publications] Hamaoka H, Arase S et al: "Effect of K^+ channel openers on K^+ channel in cultured human dermal papilla cells." J Med Invest. 44. 73-77 (1997)
[Publications] kunika K, Yamaoka Y, Itakura M: "Damage of Charge Dependent Renal Tubular Reabsorption Causes Diabetic Microproteinuria" Diabet.Res.Clin.Pract. 36. 1-9 (1997)
[Publications] Sarui H, Itakura M et al: "Cystic Glucagonoma with Lossof Heterozygosity on Chromosome 11 in Multiple Endocrine Neoplasia Type 1." Clin.Endocrinol. 46. 511-516 (1997)
[Publications] Tomonari A, Itakura M et al: "Proximal Nucleotide Sequence of the Humang-GlutamylcysteineSynthetase Heavy Subunit Gene is Involved in Cisplatin-Induced Transcriptional Up-Regulation in a Lung Cancer Cell Line SBC3." Biochem.Biophys.Res.Commun. 236. 616-621 (1997)
[Publications] Ninomiya Y, Itakura M et al: "p53 Gene Mutation Analysis in porokeratosis and porokeratosis-associated squamous cell carcinoma" J.Dermatol.Science. 14. 173-178 (1997)
[Publications] MinH et al: "p53 Gene Mutaion in N-Butyl-N-(4-hydroxybutyl)nitrosamine-Induced Urinary Bladder Tumors and N-Methyl-N-nitrosourea-induced Colon Tumors of LEC and F344 Rats" 117. 81-86 (1997)
[Publications] Yamaoka T, Itakura M et al: "Amidophosphoribosyltransferase Limits the Rate of Cell Growth-linked De Novo Purine Biosynthesis in the Presence of Constant Capacity of SalvagePurine Biosynthesis." J.Biol.Chem. 272. 17719-17725 (1997)
[Publications] Tanaka C, Itakura M et al: "Infrequent Mutations of p27Kip1 Gene and Trisomy 12 in a Subset of Human Pituitary Adenomas" J.Clin.Endocrinol.Matab. 82. 3141-3147 (1997)
[Publications] Ozaki S, Kosaka M, et al: "Thrombopoietin-responsive essential thrombocythemia with myelofibrosis" Brit I Haematol. 97. 449-452 (1997)
[Publications] Ozaki S, Kosaka M et al: "Immunotherapy of multiple myeloma with a monoclonal antibody directed against a plasma cell-specific antigenn,HM1.24" Blood. 90. 3179-3186 (1997)
[Publications] Shiozaki M, Kosaka M, Eto Y: "Activin A ; a commiment factor in erythroid differentiation" BBRC. 242. 631-635 (1998)
[Publications] 小坂昌明、尾崎修治: "ヒト形質細胞腫モデルと抗ヒト骨髄主細胞抗体の抗腫瘍効果" 臨床血液. 38. 275-280 (1997)
[Publications] 阿部正博、小坂昌明他: "多発性骨髄腫" 日本医事新報. 3833. 33-36 (1997)
[Publications] Sakuma H, Kawano Y et al.: "Risk of cytomegalovirus infection after peripheral blood stem cell transplantation" Bone-Marrow-Transplant. 19. 49-53 (1997)
[Publications] Abe T, Kawano Y et al: "Transduction of retrovirus-mediated NeoR gene into CD34_+ cells purified from granulocyte colony-stimulating factor (G-CSF)-mobilized infant and cord blood" Exp-Hematol. 25. 966-971 (1997)
[Publications] Okamoto Y, Kawano Y et al: "Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34_+ cells." Cancer-Res. 57. 5037-5040 (1997)
[Publications] Abe T, Kawano Y et al: "Effect of recombinant erythropoietin in interaction with stromal factors on cord blood hematopoiesis" Blood.87. 3212-3217 (1996)
[Publications] Scott L, Merrilees MJ et al: "Subendothelial proteoglycan synthesis and transforming growth factor beta distribution correlate with susceptibility to atherosclerosis" J-Vasc-Res. 34. 365-377 (1997)
[Publications] Poole CA, Merrilees MJ et al: "Detection of viable and non-viable cells in connective tissue explants using the fixable fluoroprobes 5-chloromethylfluorescein diacetate and ethidium homodimer-1" Connect-Tissue-Res. 33. 333-341 (1996)
[Publications] Merrilees MJ, Beaumont BW, Scott LJ: "Fluoroprobe quantification of viabie and non-viable cells in human coronary and intemal thoracic arteries sampled at autopsy" J-Vasc-Res. 32. 371-377 (1995)
[Publications] Davidson AJ, Crosier KE et al: "Expression of murine interleukin 11 and its receptor alpha-chain in adult and embryonic tissues." Stem-Cells. 15. 119-124 (1997)
[Publications] Crosier KEet al: "Expression and functional analysis of two isoforms of the human GM-CSF receptor alpha chain in myeloid development and leukaemia" Br-J-Haematol.98. 540-548 (1997)
[Publications] Crosier KE, Crosier PS: "New insights into the control of cell growth ; the role of the Axl family." Pathology. 29. 131-135 (1997)
[Publications] Lewis PM, Crosier KE et al: "Analysis of the murine Dtk gene identifies conservation of genomic structure within a new receptor tyrosine kinase subfamily" Genomics. 31. 13-19 (1996)
[Publications] Crosier PS, Crosier KE et al: "The Dtk receptor tyrosine kinase,which binds protein S,is expressed during hematopoiesis" Exp-Hematol. 24. 318-323 (1996)
[Publications] Holdaway IM, France JT et al: "Characteristics of the menstrual cycle at the time of surgery for breast cancer" Br-J-Cancer. 75. 413-416 (1997)
[Publications] Song Y, Keelan J, France JT: "Activin-A stimulates,while transforming growth factor beta 1 inhibits,chorionic gonadotrophin production and aromatase activity in cultured human placental trophoblasts." Placenta.17. 603-610 (1996)
[Publications] France JT et al: "Serum concentrations of human chorionic gonadotrophin and immunoreactive inhibin in early pregnancy and recurrent miscarriage : a longitudinal study." Aust-N-Z-J-Obstet-Gynaecol. 36. 325-330 (1996)
[Publications] Donaldxon P, Green CR et al: "Gap junctional channels : new roles in disease" Histology and Histopathology. 12. 219-231 (1997)
[Publications] Blackbum JP, Green CR et al: "Connexin 43 gap Junction levels during development of the thoracic aorta are temporally correlated with elastic laminae deposition andincreases blod pressure" Cell Biol Int. 21. 87-97 (1997)
[Publications] Walker MM, Green CR et al: "Structure and function of the vertebrate magnetic sense" Nature. 390. 371-376 (1997)
[Publications] Bond J, Green CR et al: "Liquefaction of cortical tissue in diabetic and galactosemic rat lenses defined by confocal laser scanning microscopy" Invest Opthal Visual Sci. 37. 1557-1565 (1996)
[Publications] Charles CJ, Cooper GJ et al: "Hemodynamic,hormonal,and renal effects of adrenomedullin in conscious sheep." Am-J-Physiol. 272. 2040-2047 (1997)
[Publications] Goldsbury CS, Cooper GJ et al: "Polymorphic fibrillar assembly of human amylin" J Struct Biol.119. 17-27 (1997)
[Publications] Cooper GJ, Tse-CA: "Amylin,amyloid and age-related disease" Drugs-Aging.9. 202-212 (1996)

1997 Fiscal Year Annual Research Report

細胞増殖因子、形態形成因子の細胞分化における役割

Principal Investigator

荒瀬 誠治 徳島大学, 医学部, 教授 (90108887)

Research Products

[Publications] Takiwaki H, Arase S et al: "Computor simulation of skin color based on a multilayered skin model" Skin Res Technol. 3. 36-41 (1997)

[Publications] Takiwaki H, Arase S et al: "A simple method to estimate CIE-L l.a.b.values of the skin from its videomicroscopic image" Skin Res Technol. 3. 42-44 (1997)

[Publications] Ninomiya Y, Arase S et al: "P53 gene mutation analysis in porokeratosis and porokeratosis-associated squamous cell carcinoma" J Dermatol. 14. 172-178 (1997)

[Publications] Kubo Y, Arase S et al: "Mutations of the INK4a locus in squamous cell carcinomas of human skin" BBRC. 232. 38-41 (1997)

[Publications] Shikiji T, Arase S et al: "A case of episodic angioedema associated with eosinophilia" J Med Invest. 44. 103-108 (1997)

[Publications] Miyamoto H, Arase S et al: "Color analysis of nevus of Ota for evaluation of treatment with a Q-switched alexandrite laser." Skin Res Technol. 3. 45-48 (1997)

[Publications] Miyaoka Y, Arase S et al: "A case of dermatomyositis complicated by thrombotic thrombocytepenic purpura" Dermatology. 194. 68-71 (1997)

[Publications] Hamaoka H, Arase S et al: "Effect of K^+ channel openers on K^+ channel in cultured human dermal papilla cells." J Med Invest. 44. 73-77 (1997)

[Publications] kunika K, Yamaoka Y, Itakura M: "Damage of Charge Dependent Renal Tubular Reabsorption Causes Diabetic Microproteinuria" Diabet.Res.Clin.Pract. 36. 1-9 (1997)

[Publications] Sarui H, Itakura M et al: "Cystic Glucagonoma with Lossof Heterozygosity on Chromosome 11 in Multiple Endocrine Neoplasia Type 1." Clin.Endocrinol. 46. 511-516 (1997)

[Publications] Tomonari A, Itakura M et al: "Proximal Nucleotide Sequence of the Humang-GlutamylcysteineSynthetase Heavy Subunit Gene is Involved in Cisplatin-Induced Transcriptional Up-Regulation in a Lung Cancer Cell Line SBC3." Biochem.Biophys.Res.Commun. 236. 616-621 (1997)

[Publications] Ninomiya Y, Itakura M et al: "p53 Gene Mutation Analysis in porokeratosis and porokeratosis-associated squamous cell carcinoma" J.Dermatol.Science. 14. 173-178 (1997)

[Publications] MinH et al: "p53 Gene Mutaion in N-Butyl-N-(4-hydroxybutyl)nitrosamine-Induced Urinary Bladder Tumors and N-Methyl-N-nitrosourea-induced Colon Tumors of LEC and F344 Rats" 117. 81-86 (1997)

[Publications] Yamaoka T, Itakura M et al: "Amidophosphoribosyltransferase Limits the Rate of Cell Growth-linked De Novo Purine Biosynthesis in the Presence of Constant Capacity of SalvagePurine Biosynthesis." J.Biol.Chem. 272. 17719-17725 (1997)

[Publications] Tanaka C, Itakura M et al: "Infrequent Mutations of p27Kip1 Gene and Trisomy 12 in a Subset of Human Pituitary Adenomas" J.Clin.Endocrinol.Matab. 82. 3141-3147 (1997)

[Publications] Ozaki S, Kosaka M, et al: "Thrombopoietin-responsive essential thrombocythemia with myelofibrosis" Brit I Haematol. 97. 449-452 (1997)

[Publications] Ozaki S, Kosaka M et al: "Immunotherapy of multiple myeloma with a monoclonal antibody directed against a plasma cell-specific antigenn,HM1.24" Blood. 90. 3179-3186 (1997)

[Publications] Shiozaki M, Kosaka M, Eto Y: "Activin A ; a commiment factor in erythroid differentiation" BBRC. 242. 631-635 (1998)

[Publications] 小坂 昌明、尾崎 修治: "ヒト形質細胞腫モデルと抗ヒト骨髄主細胞抗体の抗腫瘍効果" 臨床血液. 38. 275-280 (1997)

[Publications] 阿部 正博、小坂 昌明 他: "多発性骨髄腫" 日本医事新報. 3833. 33-36 (1997)

[Publications] Sakuma H, Kawano Y et al.: "Risk of cytomegalovirus infection after peripheral blood stem cell transplantation" Bone-Marrow-Transplant. 19. 49-53 (1997)

[Publications] Abe T, Kawano Y et al: "Transduction of retrovirus-mediated NeoR gene into CD34_+ cells purified from granulocyte colony-stimulating factor (G-CSF)-mobilized infant and cord blood" Exp-Hematol. 25. 966-971 (1997)

[Publications] Okamoto Y, Kawano Y et al: "Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34_+ cells." Cancer-Res. 57. 5037-5040 (1997)

[Publications] Abe T, Kawano Y et al: "Effect of recombinant erythropoietin in interaction with stromal factors on cord blood hematopoiesis" Blood.87. 3212-3217 (1996)

[Publications] Scott L, Merrilees MJ et al: "Subendothelial proteoglycan synthesis and transforming growth factor beta distribution correlate with susceptibility to atherosclerosis" J-Vasc-Res. 34. 365-377 (1997)

[Publications] Poole CA, Merrilees MJ et al: "Detection of viable and non-viable cells in connective tissue explants using the fixable fluoroprobes 5-chloromethylfluorescein diacetate and ethidium homodimer-1" Connect-Tissue-Res. 33. 333-341 (1996)

[Publications] Merrilees MJ, Beaumont BW, Scott LJ: "Fluoroprobe quantification of viabie and non-viable cells in human coronary and intemal thoracic arteries sampled at autopsy" J-Vasc-Res. 32. 371-377 (1995)

[Publications] Davidson AJ, Crosier KE et al: "Expression of murine interleukin 11 and its receptor alpha-chain in adult and embryonic tissues." Stem-Cells. 15. 119-124 (1997)

[Publications] Crosier KEet al: "Expression and functional analysis of two isoforms of the human GM-CSF receptor alpha chain in myeloid development and leukaemia" Br-J-Haematol.98. 540-548 (1997)

[Publications] Crosier KE, Crosier PS: "New insights into the control of cell growth ; the role of the Axl family." Pathology. 29. 131-135 (1997)

[Publications] Lewis PM, Crosier KE et al: "Analysis of the murine Dtk gene identifies conservation of genomic structure within a new receptor tyrosine kinase subfamily" Genomics. 31. 13-19 (1996)

[Publications] Crosier PS, Crosier KE et al: "The Dtk receptor tyrosine kinase,which binds protein S,is expressed during hematopoiesis" Exp-Hematol. 24. 318-323 (1996)

[Publications] Holdaway IM, France JT et al: "Characteristics of the menstrual cycle at the time of surgery for breast cancer" Br-J-Cancer. 75. 413-416 (1997)

[Publications] Song Y, Keelan J, France JT: "Activin-A stimulates,while transforming growth factor beta 1 inhibits,chorionic gonadotrophin production and aromatase activity in cultured human placental trophoblasts." Placenta.17. 603-610 (1996)

[Publications] France JT et al: "Serum concentrations of human chorionic gonadotrophin and immunoreactive inhibin in early pregnancy and recurrent miscarriage : a longitudinal study." Aust-N-Z-J-Obstet-Gynaecol. 36. 325-330 (1996)

[Publications] Donaldxon P, Green CR et al: "Gap junctional channels : new roles in disease" Histology and Histopathology. 12. 219-231 (1997)

[Publications] Blackbum JP, Green CR et al: "Connexin 43 gap Junction levels during development of the thoracic aorta are temporally correlated with elastic laminae deposition andincreases blod pressure" Cell Biol Int. 21. 87-97 (1997)

[Publications] Walker MM, Green CR et al: "Structure and function of the vertebrate magnetic sense" Nature. 390. 371-376 (1997)

[Publications] Bond J, Green CR et al: "Liquefaction of cortical tissue in diabetic and galactosemic rat lenses defined by confocal laser scanning microscopy" Invest Opthal Visual Sci. 37. 1557-1565 (1996)

[Publications] Charles CJ, Cooper GJ et al: "Hemodynamic,hormonal,and renal effects of adrenomedullin in conscious sheep." Am-J-Physiol. 272. 2040-2047 (1997)

[Publications] Goldsbury CS, Cooper GJ et al: "Polymorphic fibrillar assembly of human amylin" J Struct Biol.119. 17-27 (1997)

[Publications] Cooper GJ, Tse-CA: "Amylin,amyloid and age-related disease" Drugs-Aging.9. 202-212 (1996)

荒瀬誠治徳島大学, 医学部, 教授 (90108887)

[Publications] 小坂昌明、尾崎修治: "ヒト形質細胞腫モデルと抗ヒト骨髄主細胞抗体の抗腫瘍効果" 臨床血液. 38. 275-280 (1997)

[Publications] 阿部正博、小坂昌明他: "多発性骨髄腫" 日本医事新報. 3833. 33-36 (1997)