1998 Fiscal Year Final Research Report Summary
Development of Methods for Insect Viruses
| Project/Area Number |
08306004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
蚕糸・昆虫利用学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGATA Masao The University of Tokyo, Graduate School of Agricultural and Life Science, Associate Professor, 大学院・農学生命科学研究所, 助教授 (70107407)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Jun Mie University, Faculty of Engineering, Assistant Professor, 工学部, 助手 (70242930)
KAWASAKI Hideki Utsunomiya University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (00134263)
KUNIMI Yasuhisa The University of Agriculture and Technology, Faculty of Agriculture, Associate, 農学部, 助教授 (50195476)
NAKAGAKI Masao Shinshu University, Faculty of Textile, Associate Professor, 繊維学部, 助教授 (70135169)
KOBAYASHI Michihiro Nagoya University, School of Agricultural Sciences, Professor, 大学院・生命農学研究, 教授 (60111837)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Insect / Insect Viruses / Virus Infection |
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| Research Abstract |
1. An atomic force microscope was applied to observation of insect viruses. Various methods for sample preparation were examined and an easy and rapid preparation of nuclear polyhedrosis virus was developed. 2. The proteins that appeared in the silkworm after infection of densonucleosis virus were analyzed and expression of them was revealed to be specific to the virus infection though they were uncoded to the virus genome. 3. Transcription products of densonucleosis were analyzed after developing a new method with recombination of their ORF and baculovirus vector. 4. Relationships between entomopox virus and parasitic insects in a host insect were examined and it was shown that virus infection affected on development of the parasitic insect. 5. An assay system of cell growth factors in the silkworm hemolymph that was based on in vitro cultivation of wing discs was established. 6. Genes of cytoplasmic polyhedrosis virus were expressed in the cultivated cells with using baculovirus vector system and specific antiserum to peptides coded in segment 8th and 9th was prepared for investigation of the virus replication.
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