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1998 Fiscal Year Final Research Report Summary

Studies on development of techniques for isolating virus resistance genes in tomato

Research Project

Project/Area Number 08456003
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionOkayama University

Principal Investigator

MOTOYOSHI Fusao  Okayama University, Research Institute for Bioresources, Professor, 資源生物科学研究所, 教授 (90230052)

Co-Investigator(Kenkyū-buntansha) OGURA Yutaka  Okayama University, Research Institute for Bioresources, Research Associate, 資源生物科学研究所, 助手 (60224193)
MURATA Minoru  Okayama University, Research Institute for Bioresources, Associate Professor, 資源生物科学研究所, 助教授 (20166292)
Project Period (FY) 1996 – 1998
KeywordsTomato mosaic virus / Resistance gene / RAPD / SCAR / Near isogenic line / PCR / Differential display / cDNA
Research Abstract

1.DNA markers nearest the Tm-1 and Tm-2 loci
The linear order of the known six DNA markers (SCAR markers) linked to the Tm-1 locus was not able to be determined, since they did not segregate m . progeny of a hybrid between near-isogenic tomato lines, GCR26 (+/+) and GC R237 ( Tm-2/Tm-2) . In another approach, we checked four Lycopersicon hirsutum lines. As a result, I101100 was estimated to be the nearest marker of the Tm-1 locus.
Similarly, we attempted to determine the linear order of the known nine DNA markers (RAPD or SCAR markers) by comparing four tomato lines which have Tm-2 or one of the alleles of Tm-2. As a result, Nl3_<1000>, I13_<1300>, E16900 and, G09700 was estimated to be nearest the Tm-2 locus.
2. Search for disease resistance gene-like sequences in near-isogenic tomato lines
Comparing patterns of PCR products and Southern hybridizatin patterns betwee the near-isogenic lines, we examined whether there are sequences different between near-isogenic lines, but failed to find any difference betweenthem.
On the other hand, we found that there are a great number of resistance gene-like sequences, and was able to estimate a differentiation process of one sequence to .two differnt sequences.
3. Detection of cDNA sequences specific to a ToMV resistant line
We isolated cDNA fragments differentially displayed between GCR26 (+/+) and GCR236 (Tm-2/Tm-2) and determined their sequences. As a result, we found two fragments specific to GCR236, one of which has a sequence similar to apart of a gene encoding xyloglucan endo-transglycolase of tomato, and the other to a part of a gene encoding a protein kinase.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Motoyoshi, F.: "Molecular characterization of heterochromatic regions around the Tm-2 locus in chromosome 9 of tomato" Symposia on the Society for Experimental Botany. 50. 65-70 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohmori, T.: "Characterization of diease resistance gene-like sequences in near-isogenic lines of tomato" Teoretical and Applied Genetics. 96. 331-338 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyoshi, F., Ohmori, T.and Murata, M.: "Molecular characterization of heterochromatic regions around the Tm-2 locus in chromosome 9 of tomato" Symposia on the Society for Experimental Botany. 50. 65-70 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohmori, T., Murata, M.and Motoyoshi, F.: "Characterization of diease resistance gene-like sequences in near-isogenic lines of tomato" Teoretical and Applied Genetics. 96. 331-338 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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