1997 Fiscal Year Final Research Report Summary
Analysis of prion・gene ptomoter region using immortalized prionless cells
| Project/Area Number |
08456158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ONODERA Takashi Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Professor, 大学院・農学生命科学研究科, 教授 (90012781)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Yasunobu Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Assistant Professor, 大学院・農学生命科学研究科, 助教授 (90251420)
MATSUMOTO Yoshitugu Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Associate Professor, 大学院・農学生命科学研究科, 助教授 (00173922)
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| Project Period (FY) |
1996 – 1997
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| Keywords | prion / mouse / gene targeting / gene / apoptosis / promoter / adenovirus / PCR |
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| Research Abstract |
Prions cause degenerative disease of the central nervous system in both humans and animals and are composed of the scrapie isoform of the prion protein (PrP^<SC>) (Prusiner, 1991 ; Chesebro et al., 1985 ; Oesch et al., 1985 ; Kretzschmar et al., 1986). The chromosomal PrP gene encodes both PrP^C and cellular isoform, PrP^C. The entire open reading frame (ORF) is contained within a single exon, which was considered to be separated from one or two exons encoding the 5' untranslated region of PrP mRNAs by a large intron. The cell surface localization of PrP^C together with its highly expression in the brains of neonatal hamsters suggested that PrP^C might function in cell recognition (Stahl et al., 1987 ; Mobley et al., 1998). Of particular interest are 5' upstream of a untranslated regions of so-called exon 1, which will work as a promoter for the transcription of PrP mRNA.These upstream region will help us to uncover the unknown promoter/enhancer system for PrP gene.
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Research Products
(12 results)
