1997 Fiscal Year Final Research Report Summary
The role of colloid particle to reduce the volume of third space in rat surgical stress model
Project/Area Number |
08457414
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Anesthesiology/Resuscitation studies
|
Research Institution | SAITAMA MEDICAL SCHOOL |
Principal Investigator |
MIYAO Hideki Saitama Medical School, Anesthesiology, Associate Professor, 医学部, 助教授 (40129680)
|
Co-Investigator(Kenkyū-buntansha) |
KAKOI Hiroko Kitasato Medical Center, Anesthesia, MD, 麻酔科, 医員
SEKIGUTI Hiroyuki Saitama Medical School, Anesthesiology, MD (Tutor), 医学部, 助手
|
Project Period (FY) |
1996 – 1997
|
Keywords | Co11oid / Plasma Substitute / Third Space / Ischemia-reperfusion / animal model / intravenous fluid / Pentastarch / Hydoroxyethyl Starch |
Research Abstract |
Introduction : Sealing endotherial gap with large molecular sized co11oids may contribute to reduce the formation of interstitial edema according to surgical stress. The purpose of this study was to investigate which size of co11oid could reduce an edema formation in an ischemia-repurfusion model in rat. Methods : Thirty eight rats were allocated into four groups ; Normal saline (NS) group (n=10), 6% Hydroxyethyl Starch (HES) group (n=10, average of weighted molecular weight is 70,000), 6% Pentastarch (PS) group (n=9, average of weighted molecular weight is 28,000), and 5% Albumine (ALB) group (n=9, molecular weight is 69,000). After the left hind limb turniquet with a rubber band for 3 hours, the animal was anesthetized with halothane and 0.015 m1/g of each fluid was injected intravenous1y, and then the ischemic hind limb was reperfused by cutting the rubber band. Twenty four hours later, the animal was anesthetized and killed. Gastrocunemius muscles of both control and ischemic leg were removed and ovened to mesure the water content. A part of the muscle was fixed with formaline for PAS staining and was examined with a light-microscope. Results and Discussion : The morphological examination revealed that there was an interstitial edema and extra vasation of lots of co11oid particles. The insulted muscle showed a considerable edema formation. Although the water content of insulted muscle was about 1.8 times more than that of control muscle, there wag no statistical difference among the four groups. These findings may lead to an idea that the three hours ischemia was too severe insult to differenciate the effects of co11oid infusion. Conclusion : (1) The three hours ischemia and reperfusion injury to rat hind limb led to an interstitial edema and extravasation of co11oid particles. (2) The large size col1oid solutions did not reduce the water content of the insulted muscle.
|