1997 Fiscal Year Final Research Report Summary
STUDY ON THE ALLERGENECITY OF MAJOR SOYBEAN ALLERGEN Gly m Bd 26K
| Project/Area Number |
08660161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食品科学・栄養科学
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| Research Institution | Okayama Prefectural University (1997)
The University of Tokushima (1996) |
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Principal Investigator |
TSUJI Hideaki Okayama Pref.Univ.Fac.Health and Welfare Science, Professor, 保健福祉, 教授 (20093875)
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| Co-Investigator(Kenkyū-buntansha) |
BANDO Noriko Univ.Tokushima, School of Medicine, Associate, 医, 教務員 (40116851)
KIMOTO Masumi Okayama Pref.Univ.Fac.Health and Welfare Science, Associated Professor, 保健福祉, 助教授 (40108866)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Sovbean / Allergen / Gly m Bd 26K / Sugar chain |
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| Research Abstract |
We had previously carried out the elucidation of the allergens in soybean using soybean-sensitive patients with atopic dermatitis. Gly m Bd 68K,30K and 26K was shown to be the major soybean allergens. No information as to Gly m Bd 26K has been reported. Therefore, in the present study, we attempted to purify the allergen and to elucidate its properties. Furthermore, a sandwich ELISA for Gly m Bd 26K has been developed and utilized for the determination of the contents of the allergen in various soybean products. Gly m Bd 26K was purified to near homogeneity from defatted soybean by various chromatographies. The purified allergen was shown to be an Asn-type glycoprotein with a molecular mass of 26 kDa. The glycosylation site of the allergen was determined to be Asn-20. Whether some part of IgE antidbo dies in the sera of the soybean-sensitive patients can recognize the sugar moiety of the allergen was examined using other plant glycoproteins such as bromelain and horseradish peroxidase and anti-the sugar moiety of the peroxidase. Some IgE antibodies in the sera could bind to the sugar moiety of Gly m Bd 26K.
In order to determine the contents of Gly m bd26K in soybean products, two monoclonal antibodies have been prepared. Using the antibodies, a sandwich ELISA for the allergen has been established. By the present method, 0.5-10ng of the allergen could be determined. Using the method, the Gly m Bd 26K contents of soybean products were assayd. The present allergen was shown to occur at high concentrations in tofu, tonyu, yuba and abura-age, but no allergen could be detected in shoyu, miso, natto and products supplemented with SPI,such as meat balls.
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