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1997 Fiscal Year Final Research Report Summary

BIOLOGICAL ASPECTS OF RESTING EGG DIAPAUSE AND HATCHING AND ITS APPLICATION TO MASS PRESERVATION TECHNOLOGY

Research Project

Project/Area Number 08660235
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNAGASAKI UNIVERSITY

Principal Investigator

HAGIWARA Atsushi  NAGASAKI UNIVERSITY,FACULTY OF FISHERIES,PROFESSOR, 水産学部, 教授 (50208419)

Project Period (FY) 1996 – 1997
KeywordsROTIFERA / RESTING EGG / PRESERVATION / DIAPAUSE / HATCHING / CANNING / Brachionus plicatilis / LARVAL REARING
Research Abstract

Diapause and hatching of the marine rotifer Brachionus plicatilis Muller resting eggs were examined. The total number of nuclei in an embryo during the internal diapause period increased from 22 on Day 2 post spawn to 39 (each n=1) on Day 6. The outer layr of embryo membrane gradually thickens from 1.2 (Day 0) to 4.0 mum (Day 8) (each n=10). Irradiation at more than 350 nm caused 1-25% hatching, but it reached 50-60% at 250-310nm light. The addition of hydrogen peroxide or prostaglandins (E1, E2 or F2 alpha) caused resting egg hatching even in darkness. The production of peroxide in seawater caused by light as well as the oxidation of fatty acid to prostaglandins inside the embryo is a possible mechanism of resting egg hatching.
The hatchability of mass produced resting eggs (stored in sea water under the dark at 4゚C) gradually decreased due to bacterial infection of the porous egg surface. The canning method preserves eggs in a dry form, after lyophilization at -40oC.When partial pressures were less than 88 kPa, percent hatching after 6 months ranged between 16 and 20%, similar to that of uncanned eggs (19% hatching), but significantly lower (9 and 11%) at 88 and 101 kpa. Even after 12 months, percent hatching remained at 35% when canning was at 61 kpa. Canned eggs that were treated with NaClO (1ppm) or NFS-Na (5ppm) showed a significantly higher percent hatching (68% and 80%, respectively) than untreated eggs.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] N.Munuswamy et al.: "Structural differences between the resting eggs of Brachionus plicatilis and Brachionus rotundiformis(Rotifera,Brachionidae):an electron microscopic study" Hydrobiologia. 318. 219-223 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Balompapueng, M.D.et al.: "Effect of chemical disinfectants on the hatching of marine rotifer resting eggs(Brachionus plicatilis)." Aquaculture Research. 28. 559-565 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Balompapueng, M.D.et al.: "Preservation of the euryhaline rotifer(Brachionus plicatilis)resting eggs by canning,method." Hydrobiologia. 358. 163-166 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Munuswamy, N., A.Hagiwara, G.Murugan, K.Hirayama and H.J.Dumont: "Structural differences between the resting eggs of Brachionus plicatilis and Brachionus rotundiformis (Rotifera, Brachionidae) : an electron microscopic study." Hydrobiologia. 318. 219-223 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Balompapueng, M.D., N.Munuswamy, A.Hagiwara and K.Hirayama: "Effect of chemical disinfectants on the hatching of marine rotifer resting eggs (Brachionus plicatilis)." Aquaculture Research. 28. 559-565 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Balompapueng, M.D., A.Hagiwara, and K.Hirayama: "Preservation of the euryhaline rotifer (Brachionus plicatilis) resting eggs by canning method." Hydrobiologia. 358. 163-166 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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