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1997 Fiscal Year Final Research Report Summary

Properties of H^+-ATPase and cloning of its gene from ruminal bacteria

Research Project

Project/Area Number 08660351
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionMeiji University

Principal Investigator

HINO Tsuneo  Meiji University, Department of agriculture, Professor, 農学部, 教授 (50012050)

Project Period (FY) 1996 – 1997
Keywordsruminal bacteria / H^+-ATPase gene / atp-mRNA
Research Abstract

A gene encoding H^+-ATPase of ruminal bacteria, Ruminococcus albus and Streptococcus bovis, was cloned and the entire operon (atp) was sequenced by the inverse PCR method. The atp operon of S.bovis was found to be approximately 6.5 kbp, which consisted of atp E (c-subunit), B (a), F (b), H (delta), A (alpha), G (gamma), D (beta), and C (epsilon) in this order. In the atp operon of R.albus, the order of atp E and B was inversed, and in addition assumed atp I (i) was included. The open reading frame was approximately 7 kbp.
Primer extension analysis was conducted for the atp operon of S.bovis with the result indicating the presence of three different species of atp-mRNA.The proportion of each mRNA species was different in S.bovis cells grown at pH 4.7 compared to those grown at pH 7.0. This suggests that the synthesis of H^+-ATPase is regulated at the transcriptional or post-transcriptional level.

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Published: 1999-03-16  

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