1997 Fiscal Year Final Research Report Summary
Properties of H^+-ATPase and cloning of its gene from ruminal bacteria
| Project/Area Number |
08660351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Meiji University |
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Principal Investigator |
HINO Tsuneo Meiji University, Department of agriculture, Professor, 農学部, 教授 (50012050)
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| Project Period (FY) |
1996 – 1997
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| Keywords | ruminal bacteria / H^+-ATPase gene / atp-mRNA |
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| Research Abstract |
A gene encoding H^+-ATPase of ruminal bacteria, Ruminococcus albus and Streptococcus bovis, was cloned and the entire operon (atp) was sequenced by the inverse PCR method. The atp operon of S.bovis was found to be approximately 6.5 kbp, which consisted of atp E (c-subunit), B (a), F (b), H (delta), A (alpha), G (gamma), D (beta), and C (epsilon) in this order. In the atp operon of R.albus, the order of atp E and B was inversed, and in addition assumed atp I (i) was included. The open reading frame was approximately 7 kbp.
Primer extension analysis was conducted for the atp operon of S.bovis with the result indicating the presence of three different species of atp-mRNA.The proportion of each mRNA species was different in S.bovis cells grown at pH 4.7 compared to those grown at pH 7.0. This suggests that the synthesis of H^+-ATPase is regulated at the transcriptional or post-transcriptional level.
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