1997 Fiscal Year Final Research Report Summary
Analysis of IgE-level regulatory gene involving protection against helminth infections.
| Project/Area Number |
08670292
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
WATANABE Naohiro Jikei University School of Medicine Department of Tropical Medicine, Associate Professor, 医学部, 助教授 (00057019)
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| Project Period (FY) |
1996 – 1997
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| Keywords | IgE / genetic control / parasite / protective immunity / helminth / linkage analysis / Trichinella / atopy |
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| Research Abstract |
The objective of this study is to identify the characteristics and gene locus of IgE-level regulatory gene which controls IgE-dependent protection against helminth parasites. We previously demonstrated that strains of mice can be classified into IgE-high and -low responders under the control of IgE-level regulatory gene. Backcross (N2) mice were obtained by breeding between high and low responders. After infection with Trichinella spiralis, IgE production in N2 mice segregated into IgE-high and low responders with the ratio of 1 : 1, confirming a single autosomal gene. The numbers of T.spiralis recovered from IgE-high N2 mice were significantly smaller than those from IgE-low N2 mice. This results suggest that IgE-level regulatory gene controls protection against T.spiralis. The linkage analysis between IgE levels in N2 mice and microsatellite markers on chromosomes is in progress. The second experiments were carried out in NC/Nga mice with hyper IgE.The trait of hyper IgE is IgE-isotype specific and antigen-nonspecific, suggesting a novel IgE-level regulatory gene. In the crossing experiments, the ratio between mice with hyper IgE and with normal were 1 : 15 in F2 generation and 1 : 3 in N2 generation. These results suggest that the hyper IgE is controlled by two autosomal genes with recessive trait. We proposed the gene symbols ieh1 and ieh2 for the genes.
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