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1997 Fiscal Year Final Research Report Summary

Development of novel experimental systems for persistent Epstein-Barr virus infection

Research Project

Project/Area Number 08670351
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionNihon University

Principal Investigator

FUJIWARA Shigeyoshi  Nihon University School of Medicine Lecturer, 医学部, 講師 (30173488)

Co-Investigator(Kenkyū-buntansha) ONO Yasushi  Nihon University School of Medicine Professor, 医学部, 教授 (30004675)
Project Period (FY) 1996 – 1997
KeywordsEpstein-Barr virus / Persistent infection / Recombinant virus / Hygromycin / MT-2 / T-cell lymphoma
Research Abstract

Epstein-Barr virus (EBV) is implicated in the pathogenesis of a number of human malignancies, including Burkitt's lymphoma, nasopharyngeal carcinoma, Hodgkin's disease, peripheral T-cell lyphome, and gastric carcinoma. In spite of its association with malignancies of various histological lineages, experimental EBV infection has been extremely difficult with B-lymphocytes being the only exception, in which in vitro EBV infection easily establishes persistent infection. In this project, we attempted to establish new experimental systems of persistent EBV infection of non-B-cell lineages, by using recombinant EBV with a positive selection marker. We isolated EBV-infected clones of a human T-cell line MT-2 and characterized their EBV gene expression. The results indicated that they expressed EBV nuclear antigen 1 (EBNA1), latent membrane protein 1 (LMP1), LMP2A, BARF0, and EBER, while EBNA2 was barely detectable. Thus far, the EBV gene expression in MT-2 cells was similar to that in peripheral T-cell lymphoma. However, MT-2 cells expressed also EBNA3 and proteins of the early virus-replicative cycle, including BZLF, and was different with this respect from peripheral T-cell lymphoma. This pattern of EBV gene expression seen in MT-2 cells is clearly different from that seen in human B-cell lines BJAB and Louckes that are persistently infected with similar recombinant EBV.Thus, MT-2 cells may be useful to analyze EBV gene regulation in T-cell lineage.
In our effort to establish experimental system of persistent EBV infection in the epithelial-cell lineage, we attempted to introduce the gene encoding EBV receptor to human epithelial cell lines. However, we still have not achieved its stable and high-level expression, that is required for efficient EBV infection.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Masami Takei: "Detection of Epstein-Barr virus encoded small RNA 1 and latent membrane protein 1 in synovial lining cells from rtheumatoid arthritis patients" International Immunology. 9. 739-743 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dai Iwakiri: "Antigenic variation of the Epstein-Barr virus nuclear antigen EBNAI as revealed by monoclonal antibodies" Virus Research. 50. 139-149 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigemasa Sawada: "Soluble CD23 levels and their correlation with erythrocyte sedimentation rate and with copy number of Epstein-Barr virus genome in rheumatoid arthritis patients" Clinical Rheumatology. 16. 635-636 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤原 成悦: "Tリンパ球体における実験的EBウイルス持続感染系の確立と解析" 日本臨床. 55. 416-423 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuko Nagatsuka: "Establishment of characterization of an Epstein-Barr virus-transformed cell line,KM/C8,from a patient with infantile sialic acid storage disease(ISSD)" Biochimica et Biophysica Acta. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroyuki Nakamura: "Epstein-Barr virus-infected human T-cell line with a unique pattern of viral gene expression" International Journal of Cancer. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihiro Suzuki: "Oxidative stress triggers tyrosine phosphorylation in B cells through a redox- and inflammatory cytokine-sensitive mechanism" Immunology. 87. 396-401 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuko Nagatsuka: "The Epstein-Barr virus alters the B cell glycolipid which is recognized by the human monoclonal antibody to i-blood group antigen" Virus Research. 43. 57-68 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, H., Iwakiri, D., Ono, Y., and Fujiwara, S.: "Epstein-Barr virus-infected human T-cell line with a unique pattern of viral gene expression." Int.J.Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagatsuka, Y., Nakano, C., Nemoto, N., Jike, T., Ono, Y., and Hirabayashi, Y.: "Establishment and characterization of an Epstein-Barr-virus-transformed cell line, KM/C8, from a patient with infantile sialic acid storage disease." Biochimica et Biophysica Acta. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiwara, S., Nakamura, H., Iwakiri, D., and Ono, Y.: "Isolation and characterization of Epstein-Barr virus-infected clones of a human T-lymphpobastoid cell line" Nipponrinsho. 55. 416-423 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takei, M., Mitamura, K., Fujiwara, S., Horie, T., Ryu, J., Osaka, S., Yoshino, S., and Sawada, M.: "Detection of Epstein-Barr virus encoded small RNA land latent membrane protein 1 in synovial lining cells from rheumatoid arthritis patients." Int.Immunol.9. 739-743 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwakiri, D., Nakamura, H., Ono, Y., and Fujiwara.S.: "Antigenic variation of the Epstein-Barr virus nuclear antigen EBNA1 as revealed by monoclonal antibodies." Virus Res.50. 139-149 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawada, S., Mitamura, K,Takei, M., Okubo, T., and Fujiwara, S.: "Soluble CD23 levels and their correlation with erythrocyte sedimentation rate and with copy number of Epstein-Barr virus genome in rheumatoid arthritis patients." Clin.Rheumatol. 16. 635-636 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohtsuki, K.and Ono, Y.: "The biochemical mechanisms involved in the biological effects induced by neocarzinostatin (NCS) and the NCS chromophore." In H.Maeda, K.Edo, and N.Ishida (eds.), Neocarzinostatin : the past, present, and future of an anticancer drug.Springer-Verlag, Tokyo.129-154 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, Y., Ohsugi, K., and Ono, Y.: "Oxidative stress triggers tyrosine phosphorylation in B cells through a redox-and inflammatory cytokine-sensitive mechanism." Immunology. 87. 396-401 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagatsuka, Y., Yamaki, M., Watarai, S.et al.: "The Epstein-Barr virus alters the Bcell g lycolipid which is recognized by the human monoclonal antibody to i-blood group antigen." Virus Research. 43. 57-68 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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