1997 Fiscal Year Final Research Report Summary
Pharmacokinetics, biological effects and distribution of (1*3)-beta-D-glucan
| Project/Area Number |
08670528
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Teikyo University school of Medicine (1997)
Jichi Medical University (1996) |
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Principal Investigator |
YOSHIDA Minoru Teikyo University school of Medicine Fourth Department of Internal Medicine, Associate Professor, 医学部, 助教授 (00166977)
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| Project Period (FY) |
1996 – 1997
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| Keywords | (1*3)-beta-D-glucan / biological effects / pharmacokinetics / plasma concentration / tumor necrosis factor / lipid metabolism / ultracentrifugation / endotoxin |
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| Research Abstract |
The pharmacokinetics, biological effects and distribution in blood and organs of <@D1125@>D1I-labeled (1*3)-beta-D-glucan purified from Candida albicans were analyzed in rabbits during the 24 hr period following its I.V.administration. The intravascular half-life (T<@D21/2@>D2alpha) of beta-glucan was 1.8 min in the low dose group (9.3mug/kg) and 1.4 min in the high dose group (222mug/kg), and the total body clearance was 1.12 (]SY.+-。[) 0.30 and 1.17 (]SY.+-。[) 0.16 (ml/min, mean (]SY.+-。[) SD), respectively. The serum concentration of (1*3)-beta-D-glucan was also biologically determined by a test using coagulation factor G of the Japanese horseshoe crab (G Test). There was a good correlation between the concentration of beta-glucan, measured biologically and isotopically, during the initial 2 hr period ; however, biological activity then decreased faster than the isotopic concentration of beta-glucan during the 3-24 hr period. During the 24 hr period of observation, the rabbits remained well and beta-glucan failed to alter blood cell counts, TNF levels, and lipid metabolism. <@D1125@>D1I-beta-glucan associated with the cellular compartment initially was less than 3% (the majority in the platelets), and decreased further during the following 2 hr period (p=0.0001). Over 97% of <@D1125@>D1I-beta-glucan was associated with the cell-free plasma and the majority in plasma appeared to be present in unbound form, i.e., not associated with lipoproteins or plasma proteins. The liver contained more than 80% of the <@D1125@>D1I-beta-glucan detected in the six major organs analyzed.
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