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1997 Fiscal Year Final Research Report Summary

Application of the exo utero surgery to the study on the development of the cerebral cortical

Research Project

Project/Area Number 08680825
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKEIO UNIVERSITY

Principal Investigator

KAWAMURA Koki  KEIO UNIV., SCHOOL OF MEDICINE,DEPT.OF ANATOMY,PROFESSOR, 医学部, 教授 (40048286)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Kosei  DIVISION OF CELL BIOLOGY,NARA ISTITUTE OF SCIENCE AND TECHNOLOGY ASSISTANT, 助手 (90206946)
OHYAMA Kyoji  KEIO UNIV., SCHOOL OF MEDICINE,DEPT OF ANATOMY,ASSISTANT, 医学部, 助手 (00255423)
NOGAMI Haruo  KEIO UNIV., SCHOOL OF MEDICINE,DEPT OF ANATOMY,ASSOCIATE PROFESSOR, 医学部, 講師 (30119838)
KAWANO Hitoshi  KEIO UNIV., SCHOOL OF MEDICINE,DEPT.OF ANATOMY,ASSOCIATE PROFESSOR, 医学部, 講師 (20161341)
Project Period (FY) 1996 – 1997
Keywordsrat / cerebral cortex / exo utero surgery / development / L1 / antisense oligo DNA / TAG-1 / アンチセンスDNA
Research Abstract

In spite of the great advance of the gene technology in recent years, the mechanism of mammalian brain development is not fully known. One of the reasons is the difficulty in observing and manipulating mammalian embryos which develop in the mother's uterus. In the present study, some experimental manupilations were made to the embryonic mouse brain by using exo utero sugery to clarify the developmental mechanisms of the cerebral cortex. We have already studied the in vivo development of the rat cerebral cortex and obtained the data which indicate that molecular interactions between neural cell adhesion molecules, L1 and TAG-1, and brain-specific chondroitin sulfate proteoglycan (CSPG), neurocan and phosphacan, are involved in the pathway formation of the cerebral cortex (Fukuda et al., 1997). In the developing rat cerebral cortex, L1 and TAG-1 immunoreactions were specifically localized on thalamocortical axons and cortical efferent axons, respectively. L1-bearing thalamocortical axons preferentially travel in the subplate of the cortical anlage where neurocan, was specifically expressed. In contrast, axons immunoreactive for TAG-1 evaded the subplate and run in the intermediate zone where neurocan expression was less prominent. In addition, TAG-1-bearing axons extensively invaded regions expressing another type of CSPG,phosphacan. Although both TAG-1 and L1 have been reported to bind both neurocan and phosphacan in vitro, various pattern of interactions between neural cell adhesion molecules and CSPGs may play important roles in the pathway formation of the forebrain. Based on these observation, we made microinjection of an enzyme which degrades chondrotin sulfate, antibody ageinst L1 or antisense oligo DNA of TAG-1 into the lateral ventricle of E13 mouse embryos using exo utero surgery, and exmined the brain histologically. This system will be a useful tool to study the developmental mechanism of the mammalian brain.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Ikawa H, et al.: "Impaired expression of neural cell adhesion molecuie L1 in the extrlnsic nerve fibers in Hirschsprung's disease." J. Ped. Surg.32. 542-545 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda T, et al.: "Immunohistochemical localization of neuorocan and L1 in the formation of thalamocortical pathway, of developing rats." J. Comp. Neurol.382. 141-152 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohyama K, et al: "Localization of extracellular matrix molecules, integnns and their regulators, TGFβs, is correlated with axon pathfinding in the spinal cord of nomal and Danforth's short tail mice." Dev. Brain Res.103. 143-154 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano H, et al.: "Regenerating axons of the paraventriculo-neurohypophysial tract invaded the scar tissue that expresses extracellular matrix molecules." J. Brain Sci.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohyama K, et al.: "Coordinat expression of L1 and 6B4 proteoglycan / phosphacan correlated with the migration of mesencephalic dopaminergic neurons in mice." Dev. Brain Res.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto A, et al.: "Deficiency in protein L-isoaspartyl methyltransferase results in a tetal progressive epilepcy." J. Neurosci.(in press). - (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川野 仁、ら: "-神経組織の再生と機能再建-基礎と臨床(中脳ドーパミンニューロンの移動と接着因子)" 西村書店, 17 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikawa H,Kawano H,Takeda Y,Masuyama H,Watanabe K,Endo M,Yokoyama J,Kitajima M,Uyemura K,Kawamura K: "Impaired expression of neural cell adhesion melecule L1 in the extrinsic nerve fibers in Hirschsprung's disease" J.Ped.Surg.32. 542-545 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda T,Kawano H,Ohyama K,L1 H-P,Takeda Y,Oohira A,Kawamura K: "Immunohistochemical localization of neuorocan and L1 in the formation of thalamocortical pathway of developing rats." J.Comp.Neurol.382. 141-152 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama K,Kawano H,Kawamura K: "Localization of extracellular matrix molecules, integrins and their regulators, TGFbetas, is correlated with axon pathfinding in the spinal cord of normal and Danforth's short tail mice." Dev.Brain Res.103. 143-154 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano H,Funato K,Kawamura K: "Regenerating axons of the paraventriculo-neurohypophysial tract invaded the scar tissue that expresses extracellular matrix molecules." J.Brain Sci.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama K,Kawano H,Asou H,Fukuda T,Oohira A,Uyemura K,Kawamura K: "Coordinat expression of L1 and 6B4 proteoglycan/phosphacan correlated with the migration of mesencephalic dopaminergic neurons in mice." Dev Brain Res.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto A,Takagi H,Kitamura D,Tatsuoka H,Nakano H,Kawano H,Kurotanagi H,Yahagi Y,Kobayashi S,Koizumi K,Sakai T,Saito K,Chiba T,Kawamura K,Suzuki K,Watanabe T,Mori H,Shirasawa T: "Deficiency in protein L-isoaspartyl methyltransferase results in a fetal progressive epilepcy." J.Neurosci.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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