1997 Fiscal Year Final Research Report Summary

Changes in neuroactive substances in trigeminal motor nucleus after peripheral nerve injury

Research Project

Project/Area Number	08835023
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	咀嚼
Research Institution	HYOGO COLLEGE OF MEDICINE
Principal Investigator	WADA Yo HYOGO COLLEGE OF MEDICINE, Lecturer, 医学部, 助手 (20104234)
Co-Investigator(Kenkyū-buntansha)	TSUKAMOTO Yoshihiko ASSISTANT PROF., 医学部, 講師 (20104250) NOGUCHI Koichi PROFESSOR, 医学部, 教授 (10212127)
Project Period (FY)	1996 – 1997
Keywords	trigeminal motor nucleus / calcitonin gene-related peptide / in situ hybridization / masseteric nerve / peripheral nerve injury
Research Abstract	The aim of this study was to examine the change in alpha and beta calcitonin gene related peptide mRNA expression in trigeminal motor nucleus following peripheral nerve injury. Rats received the transection of left masseteric nerve under deep anesthesia. After 1-56 postoperative days, the brainstem was dissected out for in situ hybridization. Trigeminal motor nucleus showed beta-CGRP mRNA expression constitutively and no alpha-CGRP mRNA.After transection of unilateral masseteric nerve, trigeminal motor nucleus express alpha-CGRP mRNA dramatically and continued high level at least 8 weeks after nerve injury. beta-CGRP mRNA showed small increase compared to alpha-CGRP mRNA.These data suggest that trigeminal motor nucleus showed differential expression of alpha-and beta-CGRP mRNA after peripheral nerve injury.

(4 results)