1997 Fiscal Year Annual Research Report

骨形成蛋白(BMP-2)の生涯的役割-個体発生から老化まで-

Research Project

Project/Area Number	08877269
Research Institution	Niigata University
Principal Investigator	小澤英浩新潟大学, 歯学部, 教授 (60018413)
Co-Investigator(Kenkyū-buntansha)	網塚憲生新潟大学, 歯学部, 助手 (30242431) 入江一元新潟大学, 歯学部, 助手 (70223352)
Keywords	骨形成蛋白 / トノニスフォーミンク増殖因子 / 脊柱靱帯 / 歯槽骨 / レセプター / 骨芽細胞 / 軟骨細胞 / 線維芽細胞
Research Abstract	骨形成蛋白(BMP-2)の生涯的役割を包括的に解明するために、昨年度はマウス成獣の腰椎脊柱靱帯にBMP-2を用いて異所性骨化を誘導する研究や、成獣ラット上顎の抜歯窩にBMP-2を投与する実験を行い、靱帯線維芽細胞がBMP-2の投与により線維軟骨様軟骨細胞を経て硝子軟骨様軟骨細胞へ分化し内軟骨骨化により骨へ置換される現象や、抜歯窩の骨形成が促進される事を明らかにした。本年度は、BMP-2による骨形成現象に関わるサイトカインネットワークを解明する事が目標であったため、これらの実験系を用いてBMP-2やTGF-βなどのリガンドやレセプターを免疫組織細胞化学的に検索した。その結果、BMP-2により誘導される骨化現象において、BMP標的細胞におけるBMP receptorのup-regulationや、TGF-βのauto crine/paracrineによる分化誘導が起こる事が明らかとなった。骨形成蛋白が肢芽発生にも重要な役割を果たすという従来の報告も考えあわせると、骨形成蛋白は他のサイトカインとも密接な連係を保ちながら生涯にわたり骨の形成や再生、組織の形態維持に重要な役割を担っていると思われた。これらの結果および関連所見は、国際雑誌Bone Acta Hist ochemi ca et Cytochemica Histochemistry and Cell Biology等で出版された他、第102回日本解剖学会、第15回日本骨代謝学会、第18回米国骨代謝学会、第12回日本整形外科基礎学術集会、平成9年度新潟歯学会第1回定例会等で公表し、高い評価を受けた。

Research Products
(16 results)

All Other

All Publications (16 results)

[Publications] Amizuka,N.他: "Cell-specific expression of the parathyroid hormone/(PTH)/PTH-related peptide receptor gene in kidney from kidney-specific and ubiquitous promoters." Endocrinology. 138・1. 469-481 (1997)
[Publications] Rakiba,S.他: "Histochemical study of alveolar bone remodeling in op/op mice by the administration of macrophage-colony stimulating factor." J.Bone Miner.Metab.15・2. 59-66 (1997)
[Publications] Tanaka,M.他: "Histochemical examination of ectopic bone formation induced in rat bone marrow." J.Bone Miner.Metab.15・2. 67-76 (1997)
[Publications] Ohashi,N.他: "Changes in type I, II and X collagen immunoreactivity of the mandibular condylar cartilage in a naturally aging rat model." J.Bone Miner.Metab.15・2. 77-83 (1997)
[Publications] Hoshi,K.他: "Immunolocalization of tissue non-specific alkaline phosphatase in mice." Histochem.Cell Biol.107. 183-191 (1997)
[Publications] Nakamura,H.他: "Immunolocalization of CD44 and the ezrin-radixin-moesin (ERM) family in the stratum intermedium and papillary layer of the mouse enamel organ." J.Histochem.Cytochem.45・11. 1481-1492 (1997)
[Publications] Hoshi,K.他: "Fibroblasts of spinal ligaments pathologically differentiate into chondrocytes induced by recombinant human bone morphogenetic protein-2 : Morphological examinations for ossification of spinal ligaments." Bone. 21・2. 155-162 (1997)
[Publications] Amizuka,N.他: "An Ultrastructural study of cell-cell contact between mouse spleen cells and calvaria-derived osteoblastic cells in a co-culture system for osteoclast formation." Acta Histochem.Cytochem.30・4. 351-362 (1997)
[Publications] Nakamura,H.他: "The Localization of CD44 and Moesin in Osteoclasts after Calcitonin Administration in Mouse Tibiae." J.Bone Miner.Metab.15. 184-192 (1997)
[Publications] Hoshi,K.他: "Ultrastructure and immunolocalization of transforming growth factor-β in chondrification of murine ligamentous fibroblasts and endochondral calcification induced by recombinant human bone morphogenetic protein-2." Acta Histochem.Cytochem.30・4. 371-379 (1997)
[Publications] Nakamura,H.他: "CD44 and the ERM family in bone cells." Acta Histochem.Cytochem.30・2. 125-134 (1997)
[Publications] 網塚憲生他: "軟骨の分化増殖における形態学的、細胞生物学的知見" THE BONE. 11・3. 39-52 (1997)
[Publications] 小澤英浩: "骨組織の微細構造と機能に関する最近の知見" 日本界面医学会雑誌. 28. 1-12 (1997)
[Publications] Amizuka,N.他: "Morphological examination of bone synthesis via direct administration of basic fibroblast growth factor into rat bone marrow." Microscopy Research and Technique. (in press).
[Publications] 中村浩彰他: "骨シグナルと骨粗鬆症" 羊土社, 108 (1997)
[Publications] 小澤英浩: "最新骨粗鬆症" ライフサイエンス出版(印刷中),

1997 Fiscal Year Annual Research Report

骨形成蛋白(BMP-2)の生涯的役割-個体発生から老化まで-

Principal Investigator

小澤 英浩 新潟大学, 歯学部, 教授 (60018413)

Research Products

[Publications] Amizuka,N.他: "Cell-specific expression of the parathyroid hormone/(PTH)/PTH-related peptide receptor gene in kidney from kidney-specific and ubiquitous promoters." Endocrinology. 138・1. 469-481 (1997)

[Publications] Rakiba,S.他: "Histochemical study of alveolar bone remodeling in op/op mice by the administration of macrophage-colony stimulating factor." J.Bone Miner.Metab.15・2. 59-66 (1997)

[Publications] Tanaka,M.他: "Histochemical examination of ectopic bone formation induced in rat bone marrow." J.Bone Miner.Metab.15・2. 67-76 (1997)

[Publications] Ohashi,N.他: "Changes in type I, II and X collagen immunoreactivity of the mandibular condylar cartilage in a naturally aging rat model." J.Bone Miner.Metab.15・2. 77-83 (1997)

[Publications] Hoshi,K.他: "Immunolocalization of tissue non-specific alkaline phosphatase in mice." Histochem.Cell Biol.107. 183-191 (1997)

[Publications] Nakamura,H.他: "Immunolocalization of CD44 and the ezrin-radixin-moesin (ERM) family in the stratum intermedium and papillary layer of the mouse enamel organ." J.Histochem.Cytochem.45・11. 1481-1492 (1997)

[Publications] Hoshi,K.他: "Fibroblasts of spinal ligaments pathologically differentiate into chondrocytes induced by recombinant human bone morphogenetic protein-2 : Morphological examinations for ossification of spinal ligaments." Bone. 21・2. 155-162 (1997)

[Publications] Amizuka,N.他: "An Ultrastructural study of cell-cell contact between mouse spleen cells and calvaria-derived osteoblastic cells in a co-culture system for osteoclast formation." Acta Histochem.Cytochem.30・4. 351-362 (1997)

[Publications] Nakamura,H.他: "The Localization of CD44 and Moesin in Osteoclasts after Calcitonin Administration in Mouse Tibiae." J.Bone Miner.Metab.15. 184-192 (1997)

[Publications] Hoshi,K.他: "Ultrastructure and immunolocalization of transforming growth factor-β in chondrification of murine ligamentous fibroblasts and endochondral calcification induced by recombinant human bone morphogenetic protein-2." Acta Histochem.Cytochem.30・4. 371-379 (1997)

[Publications] Nakamura,H.他: "CD44 and the ERM family in bone cells." Acta Histochem.Cytochem.30・2. 125-134 (1997)

[Publications] 網塚憲生 他: "軟骨の分化増殖における形態学的、細胞生物学的知見" THE BONE. 11・3. 39-52 (1997)

[Publications] 小澤英浩: "骨組織の微細構造と機能に関する最近の知見" 日本界面医学会雑誌. 28. 1-12 (1997)

[Publications] Amizuka,N.他: "Morphological examination of bone synthesis via direct administration of basic fibroblast growth factor into rat bone marrow." Microscopy Research and Technique. (in press).

[Publications] 中村浩彰 他: "骨シグナルと骨粗鬆症" 羊土社, 108 (1997)

[Publications] 小澤英浩: "最新骨粗鬆症" ライフサイエンス出版(印刷中),

小澤英浩新潟大学, 歯学部, 教授 (60018413)

[Publications] 網塚憲生他: "軟骨の分化増殖における形態学的、細胞生物学的知見" THE BONE. 11・3. 39-52 (1997)

[Publications] 中村浩彰他: "骨シグナルと骨粗鬆症" 羊土社, 108 (1997)