メダカ脳におけるメタスチンニューロンの新規生理機能に関する研究

2008 Fiscal Year Annual Research Report

• Project/Area Number: 08F08089
• Research Institution: The University of Tokyo
• Principal Investigator: 岡 良隆 The University of Tokyo, 大学院・理学系研究科, 教授
• Co-Investigator(Kenkyū-buntansha): SADIQUL Md. 東京大学, 大学院・理学系研究科, 外国人特別研究員
• Keywords: 神経科学 / 生理学 / 脳・神経 / 動物 / 生理活性 / ペプチド / GnRH / キスペプチン

Research Abstract

強い緑色光励起により細胞特異的に細胞死を誘発する蛍光タンパク質であるKillerRed(KR)や、酸化型では無毒なMetrodidazolを還元することによってDNAに架橋構造を造って細胞毒性を持つようになる還元酵素Nitroreductase(NTR)タンパク質をGnRHニューロンに遺伝子導入したトランスジェニックメダカの作成を平成20年度に開始したが、その遺伝子コンストラクトは完成し、既に受精卵への微量注射を行っている。現在、遺伝子導入されたメダカをスクリーニングして導入遺伝子をホモでもつトランスジェニック系統を確立しつつある。トランスジェニックメダカの系統が完成すれば、これを用いてGnRH1,2,3ニューロンを特異的に細胞破壊し、メタスチンニューロンとの相互関係や行動、生殖などがどのように変化するかを生理学的に解析することができる。機能とアミノ酸配列の異なる3種類のGnRHニューロンそれぞれにGFP蛍光タンパク質を発現させるトランスジェニックメダカは既に当研究室で作成している。この遺伝子コンストラクトを参考にして、成魚まで透明性を保つ透明メダカ系統のGnRHニューロンおよびメタスチンニューロンにGFP等の蛍光タンパク質のみならずKRやNTRを遺伝子導入したトランスジェニックメダカの作成に成功すれば、それらのニューロンを細胞特異的に破壊し、生理・行動実験によりそれぞれのニューロン間の相互作用を解析する事ができるようになり、脊椎動物における生殖の中枢制御の本質的理解につながる知見が得られる。

Research Products

• [Journal Article] Primary culture of the isolated terminal nerve-gonadotrophin releasing hormone (GnRH) neurones derived from adult teleost (dwarf gourami, Colisa lalia) brain for the study of peptide release mechanisms (2009)
  • Author(s): Abe, H., Oka, Y.
  • Journal Title: Journal of Neuroendocrinology 21 Pages: 489-505
  • Peer Reviewed
• [Journal Article] Three types of gonadotrophin-releasing hormone neurones and steroid-sensitive sexually dimorphic kisspeptin neurones in teleosts (2009)
  • Author(s): Oka, Y.
  • Journal Title: Journal of Neuroendocrinology 21 Pages: 334-338
  • Peer Reviewed
• [Journal Article] Possible role of oestrogen in pubertal increase of Kiss1/Kisspeptin expression in discrete hypothalamic areas of female rats (2009)
  • Author(s): Takase, K., Uenoyama, Y., Inoue, N., Matsui, H., Yamada, S., Shimizu, M., Homma, T., Tomikawa, J., Kanda, S., Matsumoto, H., Oka, Y., Tsukamura, H., Maeda, K.-I.
  • Journal Title: Journal of Neuroendocrinology ((印刷中)掲載確定)
  • Peer Reviewed
• [Journal Article] Identification of KiSS-1 product kisspeptin and steroid-sensitive sexually-dimorphic kisspeptin neurons in medaka (Oryzias latipes) (2008)
  • Author(s): Kanda, S., Akazome, Y., Yamamoto, N., Yamada, S., Tsukamura, H., Maeda, K., Oka, Y.
  • Journal Title: Endocrinology 149 Pages: 2467-2476
  • Peer Reviewed
• [Journal Article] Coordinated synchronization in the electrically coupled network of terminal nerve gonadotropin-releasing hormone neurons as demonstrated by double patch-clamp study (2008)
  • Author(s): Haneda, K., Oka, Y.
  • Journal Title: Endocrinology 149 Pages: 3540-3548
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of GnRH-like peptide in the brain of the cephalopod spear squid, Loligo bleekeri (2008)
  • Author(s): Amano, M., Oka, Y., Nagai, Y., Amiya, N., Yamamori, K.
  • Journal Title: General and Comparative Endocrinology 156 Pages: 277-284
  • Peer Reviewed
• [Journal Article] Identification and expression analysis of peroxisome proliferator-activated receptors cDNA in a reptile, the leopard gecko (Eublepharis macularius) (2008)
  • Author(s): Kato, K., Oka, Y., Park, M. K.
  • Journal Title: Zoological Science 25 Pages: 492-502
  • Peer Reviewed
• [Presentation] Distribution of kisspeptin neurons in the CNS, with attention also to comparative aspects of Kiss1/GPR54 system (2008)
  • Author(s): Vaudry, H., Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-20081010
• [Presentation] Primary culture system of fish GnRH neurons for the cellular physiological studies of effects of kisspeptin (2008)
  • Author(s): Abe, H., Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-20081010
• [Presentation] Quantitative expression profiling of kiss1 and its receptor in the teleost medaka (2008)
  • Author(s): Okubo, K., Kanda, S., Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-20081010
• [Presentation] Possible novel form of kisspeptin and kisspeptin receptors in the medaka, Oryzias latipes (2008)
  • Author(s): Akazome, Y., Kanda, S., Mitani, Y., Okubo, K., Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-20081010
• [Presentation] Kisspeptin and steroid-sensitive sexually dimorphic kisspeptin neurons in medaka (Oryzias latipes) (2008)
  • Author(s): Kanda, S., Akazome, Y., Yamamoto, N., Yamada, S., Tsukamura, H., Maeda, K., Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-20081010
• [Presentation] Three types of GnRH neurons and steroid-sensitive sexually dimorphic kisspeptin neurons in teleosts (2008)
  • Author(s): Oka, Y.
  • Organizer: US/Japan Neurosteroid Symposium 2008
  • Place of Presentation: Gifu
  • Year and Date: 20080908-20080911
• [Presentation] 終神経GnRHニューロンへのFMRFamide ペプチドの過分極性作用に関する電気生理学的解析 (2008)
  • Author(s): 齊藤健, 阿部秀樹, 岡良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-20080907
• [Presentation] 単一細胞電気穿孔による終神経GnRHニューロンへの外来分子導入法 (2008)
  • Author(s): 入江高行, 尾崎令, 阿部秀樹, 岡良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-20080907
• [Presentation] キンギョ嗅上皮におけるカルシウム振動 (2008)
  • Author(s): 河合喬文, 阿部秀樹, 岡良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-20080907
• [Presentation] メダカ脳内kiss1遺伝子発現は卵巣エストロゲンにより調節され生殖状態を反映する (2008)
  • Author(s): 神田真司, 赤染康久, 松永拓也, 山本直之, 山田俊児, 束村博子, 前多敬一郎, 岡良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-20080907
• [Presentation] Functional analysis of the physiology of peptidergic systems using the transgenic medaka" Neurobiology of Animal Behavior and Communication (2008)
  • Author(s): Oka, Y.
  • Organizer: 31st Annual meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo
  • Year and Date: 20080709-20080711
• [Presentation] GABAergic excitation in the terminal nerve GnRH neurons (2008)
  • Author(s): Nakane, R., Oka, Y.
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 20080709-20080711
• [Presentation] Evolutionary aspects of peptidergic nervous systems in the vertebrate brain (2008)
  • Author(s): Oka, Y.
  • Organizer: International Seminar : Evolutionary Studies in Behavioral Neuroscience
  • Place of Presentation: Yokosuka
  • Year and Date: 20080625-20080627
• [Presentation] Electrophysiological and anatomical characterization of three distinct GnRH neuronal populations in the GFP transgenic medaka (2008)
  • Author(s): Kanda, S., Okubo, K., Isomae, S., Abe, H., Kobayashi, D., Takeda, H., Aida, K., Nagahama, Y., Oka, Y.
  • Organizer: Annual Meeting Society for Neuroscience 2008
  • Place of Presentation: Washington DC, U.S.A.
  • Year and Date: 2008-11-17
• [Presentation] Primary culture of the isolated terminal nerve-gonadotropin releasing hormone neurons derived from adult teleost brain and their electrophysiological properties (2008)
  • Author(s): Abe, H., Oka, Y.
  • Organizer: Annual Meeting Society for Neuroscience 2008
  • Place of Presentation: Washington DC, U.S.A.
  • Year and Date: 2008-11-17
• [Presentation] Calcium oscillation of the olfactory non-sensory cells in the goldfish (2008)
  • Author(s): Kawai, T., Abe, H., Oka, Y.
  • Organizer: Annual Meeting Society for Neuroscience 2008
  • Place of Presentation: Washington DC, U.S.A.
  • Year and Date: 2008-11-17
• [Presentation] Coordinated synchronization in the electrically coupled network of terminal nerve GnRH (gonadotropin-releasing hormone) neurons as demonstrated by double patch-clamp study (2008)
  • Author(s): Oka, Y., Haneda, K.
  • Organizer: Annual Meeting Society for Neuroscience 2008
  • Place of Presentation: Washington DC, U.S.A.
  • Year and Date: 2008-11-17
• [Presentation] 脊椎動物に共通する生殖の中枢制御のしくみをメダカの脳から知る (2008)
  • Author(s): 岡良隆
  • Organizer: 第101回日本繁殖生物学会シンポジウム「新規生理活性因子メタスチン」
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-20
• [Presentation] Medaka brain teaches us the mechanisms of the central regulation of reproduction common to the vertebrate species (2008)
  • Author(s): Oka, Y.
  • Organizer: 第35回日本神経内分泌学会・第23回日本下垂体研究会シンポジウム「視床下部・下垂体調節機構の新展開」
  • Place of Presentation: 東京
  • Year and Date: 2008-08-28
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/naibunpi/Oka/Oka_lab_publications.html