2009 Fiscal Year Annual Research Report
ヒ素感受性ゲノム解析に基づいたアジア途上国の地下水汚染地域住民のリスク評価
Project/Area Number |
08J07871
|
Research Institution | Shimane University |
Principal Investigator |
阿草 哲郎 Shimane University, 医学部, 特別研究員(PD)
|
Keywords | ヒ素 / ゲノム / 遺伝子多型 / アジア / 環境化学 / 環境毒性学 / リスク評価 / 国際共同研究 |
Research Abstract |
本年度は、ベトナムのRed River Deltaにおけるヒ素汚染地域住民のGlutathione S-transferase(GST)スーパーファミリーの遺伝子多型とヒ素代謝能力の関係について解析した。調査地域の住民190名から尿と血液を採取した。尿中ヒ素化合物は、HPLC/ICP-MSを用いて定量した。血液からDNAを抽出し、GSTM1とGSTT1のwild/nullはallele specific multiplex PCR法で、GSTO1 E155delはCTPP法で、GSTO1 A140D、GSTO1 E208K、GSTO1 T217N、GSTO2 N142D、GSTP1 I105VはPCR-RFLP法で遺伝子多型を同定した。解析の結果、GSTO1 E155del、GSTP1 I105V、GSTM1 wild/nullと尿中ヒ素化合物濃度・組成に有意な関係が認められた。GSTO1 E155delのhetero typeは尿中As(V)濃度がwild typeよりも低値であった。GSTP1 I105Vのhetero typeは、wild typeよりも尿中As(III)/As(V)が有意に低く、逆に尿中MMA(V)/(As(III)+As(V))は有意に高かった。このことから、GSTP1 1105Vの変異はAs(V)からAs(III)への還元活性を減衰させる、ある、いは無機ヒ素のモノメチル化を促進するものと推察された。尿中DMA(V)の割合は、GSTM1のnull typeで有意に低かった。一方、本研究ではGSTO1 A140D、GSTO2 N142D、GSTT1 wild/null遺伝子多型と尿中ヒ素化合物組成との間に有意な関係はみられなかった。 現在は、別のヒ素代謝酵素であるpurine nucleoside phosphorylase(PNP)の遺伝子多型を同定しながら、ヒ素の代謝能の関係について解析を進めている。また一方で、血液からRNAを抽出し、ヒ素代謝酵素や酸化ストレス等の遺伝子発現量の定量について検討している。
|
Research Products
(26 results)
-
[Journal Article] Genetic polymorphisms in glutathione S-transferase(GST)superfamily and arsenic metabolism in residents of the Red River Delta2010
Author(s)
Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Trang, P.T.K., Viet, P.H., Tanabe, S.
-
Journal Title
Toxicology and Applied Pharmacology 242
Pages: 352-362
Peer Reviewed
-
[Journal Article] Global analysis of genetic variation in human arsenic(+3 oxidation state)methyltransferase(AS3MT)2010
Author(s)
Fujihara, J., Soejima, M., Yasuda, T., Koda, Y., Agusa, T., Kunito, T., Tongu, M., Yamada, T., Takeshita, H.
-
Journal Title
Toxicology and Applied Pharmacology 243(3)
Pages: 292-299
Peer Reviewed
-
[Journal Article] Interindividual variation in arsenic metabolism in a Vietnamese population : association with 17 single nucleotide polymorphisms in AS3MT2010
Author(s)
Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Trang, P.T.K., Viet, P.H., Tanabe, S.
-
Journal Title
Interdisciplinary Studies on Environmental Chemistry-Biological Responses to Chemical Contaminants : From Molecular to Community Level (印刷中)
-
-
[Journal Article] Contamination by trace elements in e-waste recycling sites in Bangalore, India2009
Author(s)
Ha, N.N., Agusa, T., Ramu, K., Tu, N.P.C., Murata, S., Parthasaraty, P., Bulbule, K.A., Takahashi, S., Subramanian, A., Tanabe, S.
-
Journal Title
Chemosphere 76
Pages: 9-15
Peer Reviewed
-
[Journal Article] Zinc is an essential trace element for spermatogenesis in male2009
Author(s)
Yamaguchi, S., Miura, C., Kikuchi, K., Celino, F.T., Agusa, T., Tanabe, S., Miura, T.
-
Journal Title
Proceedings of the National Academy of Sciences(PNAS) 106(26)
Pages: 10859-10864
Peer Reviewed
-
-
[Journal Article] Ethnic variation in genotype frequencies of δ-aminolevulinic acid dehydratase(ALAD)2009
Author(s)
Fujihara, J., Agusa, T., Yasuda, T., Soejima, M., Kato, H., Panduro, A., Koda, Y., Kimura-Kataoka, K., Takeshita, H.
-
Journal Title
Toxicology Letters 191
Pages: 236-239
Peer Reviewed
-
[Journal Article] Genetic polymorphism influencing arsenic metabolism in human2009
Author(s)
Takeshita, H., Fujihara, J., Agusa, T., Takahashi, S., Iwata, H., Tanabe, S.
-
Journal Title
Interdisciplinary Studies on Environmental Chemistry-Environmental Research in Asia
Pages: 191-195
-
[Presentation] Soil contamination by trace elements at e-waste recycling sites in Vietnam and India2010
Author(s)
Ha, N.N., Agusa, T., I, Tue, N.M., Minh, T.B., Minh, N.H., Itai, T., Takahashi, S., Subramanian, A., Viet, P.H., Tanabe, S.
Organizer
The First International Conference on Environmental Pollution, Restoration and Management
Place of Presentation
Ho Chi Minh City
Year and Date
20100301-20100305
-
[Presentation] Arsenic, manganese and barium contamination in groundwater and human in Vietnam2010
Author(s)
Agusa, T., Inoue, S., Kunito, T., Minh, T.B., Tu, N.P.C., Ha, N.N., Trang, P.T.K., Iwata, H., Tuyen, B.T., Viet, P.H., Tanabe, S.
Organizer
The First International Conference on Environmental Pollution, Restoration and Management
Place of Presentation
Ho Chi Minh City
Year and Date
20100301-20100305
-
[Presentation] Soil contamination by trace elements at e-waste recycling sites in Vietnam and India2009
Author(s)
Ha, N.N., Agusa, T., Tue, N.M., Minh, T.B., Minh, N.H., Itai, T., Takahashi, S., Subramanian, A., Viet, P.H., Tanabe, S.
Organizer
International Symposium on Environmental Specimen Bank -Exploring Possibility of Setting-up ESBs in Developing Countries
Place of Presentation
松山
Year and Date
20091203-20091205
-
[Presentation] Distribution and biomagnification of arsenic species in the deep-sea food web of Sagami Bay, Japan2009
Author(s)
Hayase, D., Toyoshima, S., Agusa, T., Takahashi, S., Horai, S., Itai, T., Omori, K., Nishida, S., Tanabe, S.
Organizer
International Symposium on Environmental Specimen Bank -Exploring Possibility of Setting-up ESBs in Developing Countries
Place of Presentation
松山
Year and Date
20091203-20091205
-
[Presentation] Association of inorganic arsenic metabolism with genetic polymorphisms of GST isoforms in north Vietnamese populations : utilization of stored samples in the Environmental Specimen Bank of Ehime University2009
Author(s)
阿草哲郎, 岩田久人, 藤原純子, 國頭恭, 竹下治男, Minh, T.B., Trang, P.T.K., Viet, P.H., 田辺信介
Organizer
International Symposium on Environmental Specimen Bank -Exploring Possibility of Setting-up ESBs in Developing Countries
Place of Presentation
松山
Year and Date
20091203-20091205
-
-
-
-
[Presentation] ベトナムe-waste リサイクル処理現場におけるヒトの微量元素曝露の実態2009
Author(s)
野口貴子, 板井啓明, 宝来佐和子, Nguyen Mimh Tue, 高橋真, 阿草哲郎, Tu Binh Minh, Pham Thi Kim Trang, Pham Hung Viet, Minh Nguyen Hung, 田辺信介
Organizer
第15回日本環境毒性学会・バイオアッセイ研究会合同研究発表会
Place of Presentation
東京・品川
Year and Date
20091003-20091004
-
[Presentation] Interindivdiual variation in arsenic metabolism in a Vietnamese population : association with single nucleotide polymorphisms in AS3MT2009
Author(s)
阿草哲郎, 岩田久人, 藤原純子, 國頭恭, 竹下治男, Minh, T.B., Trang, P.T.K., Viet, P.H., 田辺信介
Organizer
Biological Responses to Chemical Contaminants : from molecular to community level(招待)
Place of Presentation
Aveiro, Portugal
Year and Date
20090902-20090904
-
-
-
[Presentation] ベトナム人のヒ素曝露とその代謝遺伝子多型2009
Author(s)
阿草哲郎, 岩田久人, 藤原純子, 國頭恭, Tu Binh Minh, Pham Thi Kim Trang, Pham Hung Viet, 竹下治男, 田辺信介
Organizer
第18回環境化学討論会
Place of Presentation
つくば
Year and Date
20090609-20090611
-
-
[Book] Chemical Pollution in Indochina-Contamination Status, Ecosystem Impact and Remediation Technology2009
Author(s)
Agusa, T., Iwata, H., Inoue, S., Kubota, R., Minh, T.B., Tanabe, S.
Total Pages
200
Publisher
Tokai University Press
-
-