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1999 Fiscal Year Final Research Report Summary

Immune Responses and Roles of Cytokines at the Feto-material Interface during Successful Pregnancy

Research Project

Project/Area Number 09460141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionYamaguchi University

Principal Investigator

KISO Yasuo  Faculty of Agriculture, Yamaguchi University, Professor, 農学部, 教授 (10142374)

Co-Investigator(Kenkyū-buntansha) OKADA Toshiya  Faculty of Agriculture, Osaka Prefecture University, Assistant Professor, 農学部, 助手 (00169111)
YAMATE Jyoji  Faculty of Agriculture, Osaka Prefecture University, Associate Professor, 農学部, 講師 (50150115)
MAKITA Takashi  Faculty of Agriculture, Yamaguchi University, Professor, 農学部, 教授 (90035105)
MUKAMOTO Masaya  Faculty of Agriculture, Osaka Prefecture University, Assistant Professor, 農学部, 助手 (80231629)
KANNAN Yukiko  Faculty of Agriculture, Osaka Prefecture University, Assistant Professor, 農学部, 助手 (80264810)
Project Period (FY) 1997 – 1999
Keywordsuterine NK cell / granulated metrial gland cell / TGF-β / delayed implantation / Fas / IL-15 / IL-2 receptor β-chain / TgE26
Research Abstract

Granular lymphocytes (GLs) are common inhabitants of mammalian uteri including not only the deciduata but the nondeciduata. In women and mice, these cells are transient, appear to terminally differentiate, and are hormonally dependent lymphocytes of a natural killer (NK) cell lineage. NK cells in the peripheral blood and spleen contribute to both innate and adaptive immunity by their prompt secretion of cytokines, including IFN-γ, and lysis of virally-infected and tumor cells without previous sensitization. The differentiation and function of tissue-based NK cells, such as uterine NK (uNK) cells, remain to be fully understood. We asked : 1) Are uNK cell precursors resident in the uterus? 2)When do uNK cell precursors first appear in the uterus? 3) Which factors influence the differentiation of uNK cells? 4) What are the interactions between uNK cells and extracellular matrix (ECM)?5)Does apoptosis account for the loss of uNK cells in late pregnancy? 6) How do uNK cells play crucial roles in pregnancy success? Our results are : 1) uNK cell precursors are present within nonpregnant uterine tissue and differentiate in situ, 2) the major population is replenished by precursors from other tissues, 3) the appearance of uNK cell precursors is a postnatal event, 4) ECMs play an important role in uNK cell behavior, including migration, viability, differentiation, and regulation of cytokine gene expression, 5) the Fas/Fas ligand system is the common apoptosis mediator also in uNK cells, 6) uNK cell apoptosis in late pregnancy is an important event, 7)uNK cells have critical function in pregnancy that promote decidual health, the appropriate vascularization of implantation sites, and placental size.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.Kiso 他3名: "A Study of uNK cells in pregnant IL-2Rβ chain overexpressed transgemic mice"J.Reprod.Immunol.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Mizuno 他8名: "Role of hypothemia induced by tumor necroses factor on apoptosis and function of intlammatory neutrophils in mice"Am.J.Physiol.. 278. R157-R165 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Kusakabe 他3名: "Cell death of uNK cells in mutine placenta during pkacentation and preterm priods"J.Vet.Med.Sci.. 61. 1093-1100 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Kusakabe 他5名: "Utertne NK cells produce epidermal growth factor in the mirine pragnant uterus"J.Vet.Med.Sci.. 61. 947-949 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Kiso 他4名: "The Biology of uterine NK cells at the teto-maternal interface during successpul pregnancy"The 2nd Symposium by Organized Research Cmbination System. 7 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木曽康郎 他1名: "子宮NK細胞と妊娠"産婦人科の実際. 48. 803-810 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KISO, Y, NAMBA, Y, ITOH, M, SAITO, S: "A study of uNK cells in pregnant, IL-2 receptor β-chain overexpressed transgenic mice."J. Reprod. Immunol.,. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MIZUNO, T, KANNAN, Y, TOKUNAGA, M, MORIYAMA, M, KISO, Y, KUSAKABE, K, YAMATE, J, KIYOMIYA, K, SUGANO, T.: "Role of Hypothemia Induced by Tumor Necrosis Factor on Apoptosis and Function of Infammatory Neutrophils in Mice."AM. J. PHYSIOL.,. 278. R157-R165 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KUSAKABE, K, OKADA, T, SASAKI, F, KISO, Y: "Cell death of uterine natural killer cells in murine placenta during placentation and preterm periods."J. Vet. Med. Sci,.. 61(10). 1093-1100 (1999.)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KUSAKABE, K, OHMOTO, M, OKADA, T, MUKAMOTO, M, SASAKI, F, KISO, Y.: "Uterine NK cells produce epidermal epidermal growth factor in the murine pregnant uterus."J. Vet. Med. Sci,.. 61(8). 947-949 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KISO, Y, NAMBA, Y, Tsuzaki, T, KUSAKABE, K, SAGARA, E.: "The Biology of uterine NK cells at the feto-maternal interface during successful pregnancy."The 2nd Symposium by Organized Research Combination System.. 7 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KISO, Y.: "Uterine NK cells and pregnancy (review)."Obst. Gynecol. Practice,. 48(6)(in Japanese). 803-810 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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