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1999 Fiscal Year Final Research Report Summary

Molecular and structural analyses of hemidesmosomes

Research Project

Project/Area Number 09480192
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionNagoya University

Principal Investigator

OWARIBE Katsushi  Nagoya Univ., Sch. of Informatics and Sci., Assoc. Prof., 情報文化学部, 助教授 (90109257)

Co-Investigator(Kenkyū-buntansha) USUKURA Jiro  Nagoya Univ., Sch. of Med., and Sci., Assoc. Prof., 医学部, 助教授 (30143415)
Project Period (FY) 1997 – 1999
Keywordscell adhesion / hemidesmosome / bullous pemphigoid antigens / integrin α6β4 / HD1 / plectin
Research Abstract

The hemidesmosome (HD) is a cell-to-substrate adhesion apparatus found in stratified and complex epithelia. In this project, we have investigated molecular nature of major constituents of hemidesmosomes biochemically and structurally to characterize hemidesmosomal adhesion complex.
1. The rotary-shadowed images of purified BP180 showed a characteristic molecular shape consisting of three domains, a globular head, a central rod, and a flexible tail. Further, we demonstrated that the extracellular region of BP180 molecule was cleaved on the cell membrane and released from cell surface both in tissue and in culture. We suggested biological and clinical significance of this splitting.
We also demonstrated that when BP autoantibodies bound to BP180, the cells incorporated the immune complex, and suggested that this incorporation of BP180 results in defect of BP180 and causes blistering. In joint research projects, we reported revertant mosaicism in BP180-null patient caused by mitotic gene conversion and reliable prenatal diagnosis using fetal skin biopsy and monoclonal antibodies.
2. HD1 is a major HD constituent present in the most cytoplasmic side of HD plaques, and seems to play an important role in connecting adhesion molecules with keratin intermediate filaments. Using monoclonal antibodies, partial amino acid sequencing, partial cDNA sequencing and epitope mapping, together with the results from patients with EBS-MD, we identified HD1 as plectin, a versatile cross-linking protein. In joint research projects, we reported polarized expression of HD1 and interaction of HD1/plectin with integrin β4.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Jonkman, M. F.: "Revertant mosaicism in epidermolysis bullosa caused by mitotic gene conversion"Cell. 88. 543-551 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fontao, L.: "Polarized expression of HD1 : Relationship with the cytoskeleton in cultured human colonic carcinoma cells"Exp. Cell Res.. 231. 319-327 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitajima, Y.: "Internalization of the 180-kD bullous pemphigoid antigen as immune complexes into basal keratinocytes : an important early event in blister formation in bullous pemphigoid"Br. J. Dermatol.. 138. 71-76 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirako, Y.: "Cleavage of BP180, kDa bullous pemphigoid antigen, yields a 120 kDa collagenous extracellular polypeptide"J. Biol. Chem.. 273. 9711-9717 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirako, Y.: "Hemidesmosomes and their unique transmembrane protein BP180"Microscopy Research and Technique. 43. 207-217 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okumura, M.: "Identification of the hemidesmosomal 500 kDa protein (HD1) as plectin"J. Biochem.. 126. 1144-1150 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jonkman, M. F.: "Revertant mosaicism in epidermolysis bullosa caused by mitotic gene conversion"Cell. 88. 543-551 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fontao, L.: "Polarized expression of HD1 : Relationship with the cytoskeleton in cultured human colonic carcinoma cells"Exp. Cell Res.. 231. 319-327 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitajima, Y.: "Internalization of the 180-kD bullous pemphigoid antigen as immune complexes into basal keratinocytes : an important early event in blister formation in bullous pemphigoid"Br. J. Dermatol.. 138. 71-76 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirako, Y.: "Cleavage of BP180, a 180 kDa bullous pemphigoid antigen, yields a 120 kDa collagenous extracellular polypeptide"J. Biol. Chem.. 273. 9711-9717 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirako, Y.: "Hemidesmosomes and their unique transmembrane protein BP180"Microscopy Research and Technique. 43. 207-217 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okumura, M.: "Identification of the hemidesmosomal 500 kDa protein (HD1) as plectin"J. Biochem. 126. 1144-1150 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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