| Project/Area Number |
09556066
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAI Chieko LABORATORY ANIMAL RESEARCH CENTER, INSTITUTE OF MEDICAL SCIENCE, THE UNIVERSITY of TOKYO, 医科学研究所, 教授 (10167330)
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| Co-Investigator(Kenkyū-buntansha) |
AIDA Yoko RIKEN TSUKUBA INSTITUTE, ライフサイエンス筑波研究センター, 研究員 (50182994)
KOFUNE Tomio DEPARTMENT OF PATHOLOGY, NATIONAL INSTITUTE OF HEALTH AND WELFARE, 病理部, 主任研究官 (80142644)
SAITO Izumu LABORATORY OF MOLECULAR GENETICS, INSTITUTE OF MEDICAL SCIENCE, THE UNIVERSITY OF TOKYO, 医科学研究所, 教授 (70158913)
YAMANOUCHI Kazuya NIPPON INSTITUTE FOR BIOLOGICAL SCIENCE, 研究部, 主任研究員 (30072888)
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| Project Period (FY) |
1997 – 2000
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| Keywords | adenovirus / canine distemper / CDV / H protein / CTL |
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| Research Abstract |
The AIM of this research is the development of analysis method of immune surveillance system in various animals by using adenovirus vector to characterize cell mediated immunity surveillance system. The various results were obtained, as followings,
(1) We established the recombinant adenovirus vector encoding canine distemper virus (CDV) hemagglutinin (H) gene. We also established the method to get primary dog skin cells for the target of cytotoxic T lymphocyte (CTL) assay. It makes us possible to get the targeted cells from self-animals.
(2) We identified epitopes for cell-mediated immunity and humoral immunity in N protein of rinderpest virus. By utilizing immune-stimulating complex (ISCOM), recombinant H protein was revealed to be effective in cell mediated immunity.
(3) We identified the resistant Major Histocompatibility Complex (BoLA) class II molecules to the bovine leukemia ; BoLA-DR3 allele. The recombinant adenovirus encoding BLV genome
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