1998 Fiscal Year Final Research Report Summary
Cytochemical study on the mechanisms of expression and function of steroid hormone synthesizing enzymes.
| Project/Area Number |
09670016
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
ISHIMURA Kazunori The University of Tokushima, School of Medicine, Department of Anatomy, Professor, 医学部, 教授 (90112185)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Keywords | l7alpha-Hydroxylase / C17-20 1yase / Steroid hormones / Transcription factor / Immunohistochemistry / Electron microscope / Testis / Liver / Placenta / (in press) |
|---|
| Research Abstract |
(1) Expression of PbX1, a transcription factor of 17 alpha-hydroxylase/C17-20 lyase, was studied in the rat gonads, adrenal cortex and placenta. Only in the placenta, both the transcription factor and the enzyme were co-expressed.
(2) 17alpha-Hydroxylase/G17-20 lyase was transiently expressed in the hepatocytes of 1 to 3 week-old rats. The expression of the enzyme in the liver may be involved in the onset of puberty.
(3) Keratinocytes overexpressing 12-lipoxygenase enzyme showed structural changes of the cell nuclei. The enzyme may relate to keratinization.
Apoptotic cells in the rat testis were examined by immunohistochemistry with an antibody against single-stranded DNA and in situ terminal deoxynucleotidyl transferase staining.
The results suggested that fetal Leydig cells undergo apoptosis during the perinatal period. NADPH-diaphorase and vasoactive intestinal peptide were colocalized in the penile cavernous tissues.
(4) Prostaglandin F synthase was immunohistochemically localized in the dendrites of nerve cells in the rat central nervous system.
Prostaglandin F synthase was immunohistochemically localized in the vascular endothelial cells of the rat various tissues.
12-Lipoxygenase was immunohistochemically localized in the serotonin-containing entero-endocrine cells of the mouse gastrointestinal tract. The enzyme may be involved in the hormone release.
|
