1998 Fiscal Year Annual Research Report

肺癌で欠失する癌抑制遺伝子産物を文し標的とした遺伝子治療

Research Project

Project/Area Number	09670615
Research Institution	University of Tokushima
Principal Investigator	清水英治徳島大学, 医学部, 講師 (50187449)
Co-Investigator(Kenkyū-buntansha)	川西雅士徳島大学, 医学部, 助手 (90301316)
Keywords	肺癌 / 癌遺伝子 / 癌抑制遺伝子 / p16 rb / 遺伝子治療 / 化学療法 / 癌性胸膜炎 / シスプラチン
Research Abstract	癌性胸膜炎患者7名より胸水を採取し、癌抑制遺伝子の発現状態を検討し、胸水中有核細胞を用いたコロニー形成試験で各種遺伝子の導入効果を検討した。男性5名、女性2名で、年齢の中央値は72歳であった。組織学的には腺癌6名、小細胞癌1名であった。有核細胞20万個当たりのコロニー形成数は0より120個で、7名中2名はコロニー形成がみられず、遺伝子治療の効果判定不能であった。pcDNAp16(p16cDNAの全長を含む)またはpRC/CMVRb(rb cDNAの全長を含む)をリポソームを用いて導入をした。遺伝子導入2週後にコロニー数で判定すると評価可能5名中、p16では2名で、rbでは1名でコロニー数の有意の減少がみられた。免疫組織染色でp16陽性やrb陽性とp16、rb遺伝子治療効果との間に相関性はみられなかった。抗癌剤との併用効果の検討では、シスプラチンとp16併用でコロニー数の減少において相乗効果がみとめられたが、5-FUやビンデシンとの併用では相乗効果はみられなかった。コロニー形成試験の結果、癌性胸膜炎に対する遺伝子化学療法はp16とシスプラチンの組合せが最も適切と考えられた。

Research Products

(13 results)

All Other

All Publications (13 results)

[Publications] Moriguchi S,et al: "Exercise training restores the decreased cellular immune function in obese Zucker rats via improvement of GLUT-1 expression." J Appl Physiol. 84. 311-317 (1998)
[Publications] Moriguchi S,et al: "Decreased mitogen respnse of splenic lymphocytes in obese Zucker rats is associated with the decreased expression of glucose transporter-1(GLUT-1)" Am J Clin Nutr. 67. 1124-1129 (1998)
[Publications] Houchi H,et al: "Endothelin-1 stimulatese sodium-dependent calcium efflux from bovine adrenal chromaffin cells in culture." Int J Oncol. 12. 55-60 (1998)
[Publications] HIrose M,et al: "Expression level of G1-cyclins and cell proliferation in human cultured leukemia/lymphoma cell lines" Int J Oncol. 12. 841-846 (1998)
[Publications] Kondo K,et al: "High frequency expressions of CD44 standard and variant forms in non-small cell lung cancers,but not in small cell lung cancers." J surg Oncol. 69. 128-136 (1998)
[Publications] Yamamoto A,et al: "Infreqent presence of anti-c-Myc antibodies and of c-Myc oncoprotein in sera from lung cancer patients." Oncology. 56. 129-133 (1999)
[Publications] Takeuchi E,et al: "A case of pleomophic adenoma of the lung with multiple distant metastasesobservations on its oncogene and tumor suppressor gene expression." Anticancer Res. 18. 2015-2020 (1998)
[Publications] Ise Y,et al: "An autopsy case of cytokeratin 7-positive minute adenocarcinoma of the lung eith systemic metastases." Intern Med. 37. 766-769 (1998)
[Publications] Yosshida S,et al: "Synchronous small cell carcinoma and adenocarcinoma of the lung representingdifferent retinoblastma tumor suppressor protein status." Int J Oncol. 3. 326-329 (1998)
[Publications] Namikawa O,et al: "Analysis of antibodies to p161NK4A tumor suppressor gene products in lung cancer patients." Int J Oncol. (in press). (1999)
[Publications] Yoshimoto K,et al: "Infrequent detectable somatic mutations of the RET and glial cell line-derived neurotrophic factor(GDNF)genes in human pituitary adenomas." Endocrine J. (in press). (1999)
[Publications] 清水英治他: "内科学書、島田馨編" 中山書店, 4 (1999)
[Publications] 清水英治他: "内科学書、島田馨編" 中山書店, 5 (1999)

1998 Fiscal Year Annual Research Report

肺癌で欠失する癌抑制遺伝子産物を文し標的とした遺伝子治療

Principal Investigator

清水 英治 徳島大学, 医学部, 講師 (50187449)

Research Products

[Publications] Moriguchi S,et al: "Exercise training restores the decreased cellular immune function in obese Zucker rats via improvement of GLUT-1 expression." J Appl Physiol. 84. 311-317 (1998)

[Publications] Moriguchi S,et al: "Decreased mitogen respnse of splenic lymphocytes in obese Zucker rats is associated with the decreased expression of glucose transporter-1(GLUT-1)" Am J Clin Nutr. 67. 1124-1129 (1998)

[Publications] Houchi H,et al: "Endothelin-1 stimulatese sodium-dependent calcium efflux from bovine adrenal chromaffin cells in culture." Int J Oncol. 12. 55-60 (1998)

[Publications] HIrose M,et al: "Expression level of G1-cyclins and cell proliferation in human cultured leukemia/lymphoma cell lines" Int J Oncol. 12. 841-846 (1998)

[Publications] Kondo K,et al: "High frequency expressions of CD44 standard and variant forms in non-small cell lung cancers,but not in small cell lung cancers." J surg Oncol. 69. 128-136 (1998)

[Publications] Yamamoto A,et al: "Infreqent presence of anti-c-Myc antibodies and of c-Myc oncoprotein in sera from lung cancer patients." Oncology. 56. 129-133 (1999)

[Publications] Takeuchi E,et al: "A case of pleomophic adenoma of the lung with multiple distant metastasesobservations on its oncogene and tumor suppressor gene expression." Anticancer Res. 18. 2015-2020 (1998)

[Publications] Ise Y,et al: "An autopsy case of cytokeratin 7-positive minute adenocarcinoma of the lung eith systemic metastases." Intern Med. 37. 766-769 (1998)

[Publications] Yosshida S,et al: "Synchronous small cell carcinoma and adenocarcinoma of the lung representingdifferent retinoblastma tumor suppressor protein status." Int J Oncol. 3. 326-329 (1998)

[Publications] Namikawa O,et al: "Analysis of antibodies to p161NK4A tumor suppressor gene products in lung cancer patients." Int J Oncol. (in press). (1999)

[Publications] Yoshimoto K,et al: "Infrequent detectable somatic mutations of the RET and glial cell line-derived neurotrophic factor(GDNF)genes in human pituitary adenomas." Endocrine J. (in press). (1999)

[Publications] 清水英治他: "内科学書、島田 馨編" 中山書店, 4 (1999)

[Publications] 清水英治他: "内科学書、島田 馨編" 中山書店, 5 (1999)

清水英治徳島大学, 医学部, 講師 (50187449)

[Publications] 清水英治他: "内科学書、島田馨編" 中山書店, 4 (1999)

[Publications] 清水英治他: "内科学書、島田馨編" 中山書店, 5 (1999)