1998 Fiscal Year Final Research Report Summary
Why do anesthesia and surgical procedures deteriorate host defense ability in perioperative period?
| Project/Area Number |
09671587
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Nippon Medical School |
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Principal Investigator |
OGAWA Ryo Nippon Medical School, Professor, 医学部, 教授 (20008345)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHINO Ken Nippon Medical School, Assistant, 医学部, 助手
KIM Chol Nippon Medical School, Assistant, 医学部, 助手
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| Project Period (FY) |
1997 – 1998
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| Keywords | Surgical stress / host defense inability / lypphocytepenia / apoptosis / TNF |
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| Research Abstract |
The purpose of this study is to invetigate the mechanism by which host-defence inability has been produced in patients who underwent surgical treatment.
The findings obtained are followed ; (1)The number of lymphcytes in periperal blood decreased after surgical procedures. The compositions of lymphocytes were separated into helper t-cells (CD4) and supressort-cells (CD8) by cyteflow metry. The ratio of CD4 and CD8 (CD4/CD8) decreased significantly after upper abdominal operations.(2) The lymphocytes were separated by centrifugation and cultured in a medium. Then the nuclear chromatin was stained by an indicator pigment. Microscopic examination revealed an increased fragmentation rate of nuclear chomatin in surgical patients.(3) The lymphocytes were incubated in a medium containing fas-antibody and the ratio of labelled cells were analysed, showing an increased staining in surgical patients.(4)The periphera bloods wete taken from postoperative patients to determine hormones such as cortisol and norepinephrine, and cytokines such as IL-I and TNF.The concentration of TNF in blood correlated very well to fragmentation rate of nuclear chromatin.
Th results suggest that surgical invasion stimulate the destruction of peripheral lymphocytes by apoptotic process in postoperative perid.
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