Functional analysis of protein phosphatase in vestibulo-cereberum induced by unitateral labyrinthectomy in rats

1998 Fiscal Year Final Research Report Summary

• Project/Area Number: 09671743
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Osaka University
• Principal Investigator: TAKEDA Noriaki Osaka University Medical School, Associate Professor, 医学部, 助教授 (30206982)
• Co-Investigator(Kenkyū-buntansha): KIYAMA Hiroshi Asahikawa Medical College, Professor, 教授 (00192021)
• Project Period (FY): 1997 – 1998
• Keywords: differential display / labyrinthectomy / vestibular compensation / protein phosphatase / glutamate receptor / グルタミン酸レセプター

Research Abstract

The differential desplay method was used to identify the gene expression which was altered in the cerebellar flocculus after unilateral labyrintyectomy (UL).Total RNA from flocculi of sham-operated and labyrinthectomized rats was isolated, amplified by PCR using arbitrary primer sets and separated by electrophoresis on a polyacrylarmide gel.PCR products, of which amounts were significantly different in samples from labyrinthectomized rats and those from controls, were cut out of the gel and sequenced.One of the up-regulated products was the rat protein phosphatase 2A beta catalytic subunit mRNA and one of the down-regulated products was the rat glutamate receptor delta-2 subunit mRNA.Ilistochemical study of in situ hybridization showed that those molecules were localized in the Purkinje cell layer.In labyrinthectomized rats, UL-induced nystagmus gradually disappeared within 3days after UL.These findings suggested that changes in expression of those molecules in the floccular Purkinje cells after UL is involved in vestibular compensation.