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1998 Fiscal Year Final Research Report Summary

A STUDY OF NEURONAL CIRCUIT FOR JAW MOVEMENTS,WITH IMMUNOHISTOCHEMICAL AND IN SITU HYBRIDIZATION TECHNIQUE.

Research Project

Project/Area Number 09671856
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionKANAGAWA DENTAL

Principal Investigator

TAKAHASHI Osamu  DEPARTMENT OF ORAL HISTOLOGY KANAGAWA DENTAL COLLEGE PROFESSOR, 歯学部, 教授 (70163243)

Project Period (FY) 1997 – 1998
Keywordstrigeminal motor nucleus / neuronal circuit / neurotransmitter / rat
Research Abstract

Recently a study of glutamic acid containing neurons in the brain have been carried out with the use of glutaminase, a synthetic enzyme of glutamic acid. This study was undertaken to identify the glutamatergic neuronal cell bodies and their terminals in the trigeminal motor nucleus and the trigeminal sensory nuclei, with the use of polyclonal antibody against glutamic acid.
Female Wistar rats were used for this study. They were anesthetized deeply and were perfused with 2.0% paraformaldehyde and 0.25% glutaraldehyde. The brain stem was removed immediately and serial frozen sections were made. Immunohistochemical labeling of glutamate - immunoreactive neuronal cell bodies and varicosities were performed with fluorescein isothiocyanate (FITC), and were observed under a fluorescent microscope.
Glutamate-immunoreactive (Glu-IR) cell bodies and varicosities were detected by immunofluorescence technique. In the confine of cytoarchitechtonicaliy defined trigeminal motor nucleus (Vm), a few Glu-IR varicosities were observed on motoneurons and their proximal dendrites. Dense Glu-IR fibers were seen in the caudal subnucleus (Vc) of the spinal trigeminal nucleus (Vsp), and a number of Glu-IR celis were encountered in the trigminal main sensory nucleus (Vp) and the interpolar subnucleus (Vi) of Vsp. A number of Glu-IR cells were also observed in the parvocellular reticular nucleus (pcRN) adjacent to Vsp and around Vm.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] O Takahashi et al.: "Distribution of glutamate-immunoreactive neurons and nerve fibers in the trigeminal motor nucleus and the sensory trigeminal nuclei in rats" Bulletin of Kanagawa Dental College. 27(1). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O.Takahashi, T.Tamura, M.Iwase, H.Hayashi, K.Higashi and H.Tsuzuki: "Distribution of glutamate-immuno-reactive neurons and nerve fibers in the trigeminal motor nucleus and the sensory trigeminal nuclei in rats." Bulletin of Kanagawa Dental College. 27(1)(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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