1998 Fiscal Year Final Research Report Summary

Discrimination between Cementoblast and Odontoblast using the Immunological Surface Marker

Research Project

Project/Area Number	09671943
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Conservative dentistry
Research Institution	TOHOKU UNIVERSITY
Principal Investigator	SHOJI Shigeru Tohoku University, Dental Hospital, Lecturer, 歯学部・附属病院, 講師 (10142986)
Co-Investigator(Kenkyū-buntansha)	NEMOTO Eiji Tohoku University School of Dentistry, Assistant, 歯学部, 助手 (40292221) IIYAMA Masao Tohoku University School of Dentistry, Assistant, 歯学部, 助手 (00193152) HORIUCHI Hiroshi Tohoku University School of Dentistry, Professor, 歯学部, 教授 (00013962)
Project Period (FY)	1997 – 1998
Keywords	Discrimination / cementoblast / odontoblast
Research Abstract	To regenerate the periodontal tissue, many researches have been done. The guided tissue regeneration method attaches the membrane to a root, and makes the regenerationspace for cementum, periodontal ligament and alveolar bone. Recently the enamel protein(amelogenin), which is extracted from pig toothgerm, is applied to induce the cementoblast. In 1997 we thought that the cementoblast secrete non-collagenous protein (osteopontin, sialoprotein ). So if we found the cells near the non-collagenous protein stained byimmuno-histochemical technique, we thought those cells were cementoblast. But it was very difficult to determine the cells only by their position. In 1998 we fixed our eyes on Hertwig's epithelial sheath. At the position of the formation of tooth root, a mass of Hertwigh's epithelial cells contacts with the mesenchymal cells through the laminin. We thought these mesenchymal cells, whose have a vitamin D3receptor, were cementoblast. The observation using translating electron microscopy showed the cells contacted with Hertwig's epithelial cells, and that these cells secreted the alkalinephosphatase. But we could not find the vitamin D3 receptor on these cells. We should reconsider the criteria of cementoblast, and pay the attention to the difference of cementum between human and rat.