1998 Fiscal Year Final Research Report Summary
Analysis of the Rho small GTP-binding proteinmediated signaling cascade
Project/Area Number |
09680693
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Cell biology
|
Research Institution | HOKKAIDO UNIVERSITY (1998) Osaka University (1997) |
Principal Investigator |
TANAKA Kazuma School of Medicine, Hokkaido Univ., 医学部, 教授 (60188290)
|
Project Period (FY) |
1997 – 1998
|
Keywords | Small GTP-binding protein / Cascade / Budding yeast / Cell polarity / Rho / Actin cytoskeleton / BNI1 / BNR1 |
Research Abstract |
The small GTP-binding proteins, including Rho, are implicated in the signaling cascade which regulates actin cytoskeleton-dependent cell functions. In this study, we have studied this small GTP-binding protein cascade at a molecular level. Regulation of activation of PHO1 : We have isolated and analyzed proteins which interact with ROM1/ROM2, GDPIGTP exchange factors for RHO1. Modes of action of RHO1 : We have isolated new proteins, SPA2 and SHS1, which bind BNI1, a potential target for PHO1. SPA2 is required for the localization of RHO1, and SHS1 is involved in cyotokinesis. Modes of action of BNR1 : We have isolated an SH3 domain-containing HOF1, as a protein which bind BNI1 related protein, BNR1. Both HOF1 and BNP1 are localized at the cell division site and involved in cytokinesis. As described above, we have analyzed PHO1-mediated small GTP-binding protein cascade and obtained new findings in the cascade at a molecular level.
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Research Products
(12 results)