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1998 Fiscal Year Final Research Report Summary

Adaptive radiation of Borrelia in relation to cospeciation with vector ticks on geographical isolation

Research Project

Project/Area Number 09839038
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 自然史科学
Research InstitutionFukuyama University

Principal Investigator

FUKUNAGA Masahito  Faclty of Pharmacy and Pharmaceutical Sciences, Professor, 薬学部, 教授 (20132483)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Yukie  Faclty of Pharmacy and Pharmaceutical Sciences, Research Assistant, 薬学部, 助手 (40236328)
Project Period (FY) 1997 – 1998
KeywordsBorrelia / Tick / vector / phylogenetic analysis / adaptive evolution
Research Abstract

An internal transcribed spacer (ITS2) sequence between the 5.8S and 28S rRNA genes and mitochondrial rrs sequence were used to estimate the phyletic relationships among Ixodes spp. tick vectors of Lyme disease-causing Borrelia spirochetes in geographically isolated regions. Analysis indicates that Borrelia burgdorferi sensu lato species associated with Lyme disease are found mainly in ticks of the Ixodes ricinus species complex and adapted to their own tick vectors. Other closely related tick species are not known to transmit the Borrelia-that cause Lyme disease in humans, but they appear to have a specific association with other closely related Borrelia species. There is a high degree of concordance in the phylogenetics of Borrelia taxa and the phylogenetic relationships among Ixodes ticks, which suggests a history of cospeciation in the tick-spirochete assemblage. We also investigated genetic variations of borrelial spirochetes isolated from two geographically separated sites. Seuqnce alignment using flagellin gene and outer sufface protein gene sequences showed that two genetic groups evolved independently in two separated region from borrelial species, and adapted to their own vector ticks. These results suggest that the occurrence and distribution of Borrelia in nature are depend on the vector competence of particular tick species.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 福長将仁 ほか: "Competence of a migratory bird red-bellied thrush(Turdus chrysolaus), as an avian reservoir for the Lyme disease spirochetes in Japan" Acta Tropica. 65(1). 43-51 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福長将仁 ほか: "Borrelia recurrentis characterization and comparison with retapsing-fever, Lyme assoclated, and other Borrelia spp." International Journal of Systematic Bccteriology. 47(4). 958-968 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福長将仁 ほか: "Rapid diagnosis, of Lyme dissease : Flagellin gone-based nested polymerase chain reaction for identification of causative Borrelia species" International Journal of Infections Diseases. 2(1). 64-73 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福長将仁 ほか: "Culture-positive Lyme borreliosis" The Medical Journal of Australia. 168(10). 500-502 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小田有二, 福長将仁: "rRNAスペーサ領域に基づく微生物の同定と系統分類" 化学と生物. 36(1). 7-8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福長将仁: "Borreliaの生態とライム病の分子疫学" 日本細菌学雑誌. 54(2)印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato Y,Konishi T,Hashimoto Y,Takahashi H,Nakaya K,Fukunaga M,Nakao M: "Rapid diagnosis for Lyme disease : flagellin gene-based nested polymerase chain reaction for identification of causative Borrelia species." International Journal of Infectious Diseases. 2(1). 64-73 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto K,Sato Y,Okada K,Fukunaga M,Sato F: "Competence of a migratory bird, red-bellied thrush (Turdus chrysolaus), as an avian reservoir fot the Lyme disease spirochetes in Japan." Acta Tropica. 65(1). 43-51 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cutler SJ,Moss J,Fukunaga M,Wright DJM,Fekade D,Warrell D: "Borrelia recurrentis characterization and comparison with relapsing-fever, Lyme-associated, and other Borrelia spp." International Journal of Systematic Bacteriology. 47(4). 958-968 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hudson BJ,Stewart M,Lennox VA,Fukunaga M,Yabuki M,Anna V,Moore T,Barry R,McCorriston H: "Culture positive Lyme disease in Australia." Medical Journal of Australia. 168(10). 500-502 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oda Y,Fukunaga M.: "Phylogeny of microbial organisms using rRNA spacer region." KASEAA. 36(1). 7-8 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukunaga M.: "Ecology of Borrelia and molecular epidemiology of Lyme disease." Japanese Journal of Bacteriology. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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