1998 Fiscal Year Final Research Report Summary
Synthesis of Novel Fluorescent Carbohydrates
| Project/Area Number |
10044047
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Bioorganic chemistry
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
NISHIMURA Shin-ichiro Hokkaido University Division of Biological Sciences, Professor, 大学院・理学研究科, 教授 (00183898)
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| Co-Investigator(Kenkyū-buntansha) |
NISHI Norio Hokkaido University Graduate school of Environmental Earth Science, Professor, 大学院・地球環境科学研究科, 教授 (70001857)
LEE Yuan C. Johns Hopkins University Division of Biology, Professor, 生物学科, 教授
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| Project Period (FY) |
1998
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| Keywords | energy transfer / sialyltransferase / β-1, 4-Galactosyl transferase / UDP-Gal / CMP-NANA / fluorescence / naphthyl group / dansyl group |
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| Research Abstract |
A CMP-NANA containing fluorescence probe as an energy donor and an alkylactosaminide containing fluorescence probe as an energy acceptor was successfully synthesizedegio- and stereoselectively as substrates for sialyl transferase. These substrates showed clear emission spectrum in which emission peaks were at around 340 and 525 nm due to 2-naphthyl and dansyl emissions, respectively, when the substrates was excited at 280nm. The synthetic substrates were used as substrates for SiaT based on fluorescence energy transfer. Vmax and Km were determined as 0.119 mol/min and 0.85 M, respectively. These substrates provide tremendous advantages such as sensitivity, speed, and feasibility of continuous assay.
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