1999 Fiscal Year Final Research Report Summary
ROLE OF STEROID HORMONES ON THE CELLULAR COMMUNICATION : MOLECULAR, MORPHOLOGICAL, PHYSIOLOGICAL AND BEHAVIORAL MULTIDICIPLINARY APPROACH
| Project/Area Number |
10044314
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | KYOTO PREFECTURAL UNIVERSITY OF MEDICINE |
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Principal Investigator |
KAWATA Mitsuhiro KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, PROFESSOR, 医学部, 教授 (60112512)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Hitoshi KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (60169290)
HAYASHI Shinji TOKYO METROPOLITAN INSTITUTE FOR NEUROSCIENCE, CHIEF, 解剖発生, 参事 (20076996)
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| Project Period (FY) |
1998 – 1999
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| Keywords | SUBCELLULAR LOCALIZATION / STEROID HORMONE RECEPTORS / GFP / REAL-TIME IMAGING / LIGAND-DEPENDENT TRANSCRIPTION FACTOR / GLUCOCORTIOCOLDS / SEX HORMONE / NUCLEOCYTOPLASMIC TRANSLOCATION |
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| Research Abstract |
The subcellular distribution of the steroid hormone receptors has not yet been established and controversial : different methods showed different results, I.e. each type of receptor is in cytoplasmic or nuclear or both in the absence of the ligand. To elucidate the localization of receptors of adrenal steroids and estrogen, we carried out an immunocytochemistry with the use of specific antibodies and an imaging analysis by green fluorescent protein (GFP) chimera system. Without the ligand glucocorticoid receptor (GR) is in the cytoplasmic and mineralocorticoid receptor (MR) is both in the cytoplasmic and nuclear, whereas estrogen receptor (ER) a and b are both in the nucleus. Ligand induces the accumulation of all these receptors in the nucleus. The ligand-induced movement of these nuclear receptors from inactive states depends on each type of receptor.
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[Publications] Kawata,M., Nishi,M., Yoshida,M,, Ogawa,H,, Matsuda,K., Ito,T., Morimoto M., Ozawa,H.: "Imaging analysis of nuclear receptors of corticoids and estrogen in the brain"In : Molecular Steroidogenesis (Eds.Okamato, M.,Ishimura, Y., and Nawata,H.) Universal Academy Press Inc., Tokyo, Japan. 401-404 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Matsushita,H., Takeuchi,Y., Kosaka,K., Fushiki, S., Kawata,M., Sawada, T.: "Changes in serotonergic neurons in the brain of pyrithiamine-induced acute thiamine-deficient mice"Acta Neuropathol. 98. 614-621 (1999)
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